Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 12879..13404 Replicon plasmid unnamed1
Accession NZ_CP023903
Organism Escherichia coli strain FDAARGOS_433

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CRN16_RS26185 Protein ID WP_001159871.1
Coordinates 12879..13184 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag CRN16_RS26190 Protein ID WP_000813630.1
Coordinates 13186..13404 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRN16_RS26170 8841..10007 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
CRN16_RS26175 10595..11350 - 756 WP_000852146.1 replication initiation protein RepE -
CRN16_RS26180 12072..12878 - 807 WP_000016970.1 site-specific integrase -
CRN16_RS26185 12879..13184 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CRN16_RS26190 13186..13404 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CRN16_RS26205 13964..14194 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CRN16_RS26210 14191..14607 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
CRN16_RS26215 14682..16247 + 1566 WP_001128474.1 AAA family ATPase -
CRN16_RS26220 16232..17254 + 1023 WP_000361402.1 helicase UvrD -
CRN16_RS26230 17508..18194 - 687 Protein_24 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sitABCD / mph(A) / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A) iutA / iucD / iucC / iucB / iucA 1..101274 101274
- flank IS/Tn - - 17508..17855 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T86343 WP_001159871.1 NZ_CP023903:c13184-12879 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T86343 NZ_CP023903:c13184-12879 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT86343 WP_000813630.1 NZ_CP023903:c13404-13186 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT86343 NZ_CP023903:c13404-13186 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References