Detailed information of TA system TA086329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2785682..2786307
• Replicon: chromosome
• Accession: NZ_CP023899
• Organism: Escherichia coli strain FDAARGOS_433

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CRN16_RS15730
• Protein ID: WP_000911330.1
• Coordinates: 2785909..2786307 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: CRN16_RS15725
• Protein ID: WP_000450524.1
• Coordinates: 2785682..2785909 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN16_RS15700	2781485..2781955	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
CRN16_RS15705	2781955..2782527	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
CRN16_RS15710	2782673..2783551	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CRN16_RS15715	2783568..2784602	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
CRN16_RS15720	2784815..2785528	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CRN16_RS15725	2785682..2785909	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CRN16_RS15730	2785909..2786307	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CRN16_RS15735	2786454..2787317	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
CRN16_RS15740	2787332..2789347	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
CRN16_RS15745	2789421..2790119	+	699	WP_000679823.1	esterase	-
CRN16_RS15750	2790229..2790429	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T86329 WP_000911330.1 NZ_CP023899:2785909-2786307 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T86329 NZ_CP023899:2785909-2786307 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT86329 WP_000450524.1 NZ_CP023899:2785682-2785909 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT86329 NZ_CP023899:2785682-2785909 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33