Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2345967..2346621 Replicon chromosome
Accession NZ_CP023899
Organism Escherichia coli strain FDAARGOS_433

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag CRN16_RS13555 Protein ID WP_000244777.1
Coordinates 2346214..2346621 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CRN16_RS13550 Protein ID WP_000354046.1
Coordinates 2345967..2346233 (+) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRN16_RS13525 2341255..2341998 + 744 Protein_2181 SDR family oxidoreductase -
CRN16_RS13530 2342055..2343488 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
CRN16_RS13535 2343533..2343844 + 312 WP_001679467.1 N(4)-acetylcytidine aminohydrolase -
CRN16_RS13540 2344008..2344667 + 660 WP_000250274.1 hemolysin III family protein -
CRN16_RS13545 2344744..2345724 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
CRN16_RS13550 2345967..2346233 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CRN16_RS13555 2346214..2346621 + 408 WP_000244777.1 toxin CptA Toxin
CRN16_RS13560 2346661..2347182 - 522 WP_001055874.1 flavodoxin FldB -
CRN16_RS13565 2347294..2348190 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CRN16_RS13570 2348215..2348925 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CRN16_RS13575 2348931..2350664 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-131)

Antitoxin

(7-79)


Sequences


Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T86326 WP_000244777.1 NZ_CP023899:2346214-2346621 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T86326 NZ_CP023899:2346214-2346621 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT86326 WP_000354046.1 NZ_CP023899:2345967-2346233 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT86326 NZ_CP023899:2345967-2346233 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7


Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References