Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 39225..39826 Replicon plasmid unnamed3
Accession NZ_CP023896
Organism Escherichia coli strain FDAARGOS_433

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag CRN16_RS00885 Protein ID WP_001216045.1
Coordinates 39446..39826 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag CRN16_RS00880 Protein ID WP_001190712.1
Coordinates 39225..39446 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRN16_RS00855 34235..35872 + 1638 WP_099010527.1 acyltransferase -
CRN16_RS00860 35961..37188 + 1228 Protein_37 IS3-like element IS2 family transposase -
CRN16_RS00865 37997..38371 - 375 Protein_38 integrase core domain-containing protein -
CRN16_RS00870 38373..38588 - 216 Protein_39 DNA-binding protein -
CRN16_RS00875 38763..39152 + 390 WP_000506730.1 DNA repair protein -
CRN16_RS00880 39225..39446 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CRN16_RS00885 39446..39826 + 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
CRN16_RS00890 39831..40010 + 180 WP_001339207.1 hypothetical protein -
CRN16_RS00895 40038..41081 + 1044 WP_023356283.1 DUF968 domain-containing protein -
CRN16_RS00900 41170..41622 + 453 WP_001326849.1 late promoter-activating protein -
CRN16_RS00905 41708..42901 + 1194 WP_000219625.1 hypothetical protein -
CRN16_RS00910 42901..44385 + 1485 WP_000124150.1 terminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..83988 83988


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T86318 WP_001216045.1 NZ_CP023896:39446-39826 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T86318 NZ_CP023896:39446-39826 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT86318 WP_001190712.1 NZ_CP023896:39225-39446 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT86318 NZ_CP023896:39225-39446 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References