Detailed information of TA system TA086312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1503775..1504412
• Replicon: chromosome
• Accession: NZ_CP023859
• Organism: Bacillus velezensis strain L-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: CRH11_RS07790
• Protein ID: WP_003156187.1
• Coordinates: 1504062..1504412 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: CRH11_RS07785
• Protein ID: WP_003156188.1
• Coordinates: 1503775..1504056 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRH11_RS07765	1500140..1500739	-	600	WP_015239195.1	rhomboid family intramembrane serine protease	-
CRH11_RS07770	1500832..1501197	+	366	WP_094031521.1	holo-ACP synthase	-
CRH11_RS07775	1501362..1502369	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
CRH11_RS07780	1502486..1503655	+	1170	WP_025284272.1	alanine racemase	-
CRH11_RS07785	1503775..1504056	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
CRH11_RS07790	1504062..1504412	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
CRH11_RS07795	1504530..1505351	+	822	WP_003156182.1	STAS domain-containing protein	-
CRH11_RS07800	1505356..1505721	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
CRH11_RS07805	1505724..1506125	+	402	WP_003156178.1	anti-sigma regulatory factor	-
CRH11_RS07810	1506137..1507144	+	1008	WP_007410233.1	PP2C family protein-serine/threonine phosphatase	-
CRH11_RS07815	1507208..1507537	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
CRH11_RS07820	1507534..1508016	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
CRH11_RS07825	1507982..1508770	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
CRH11_RS07830	1508770..1509372	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T86312 WP_003156187.1 NZ_CP023859:1504062-1504412 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T86312 NZ_CP023859:1504062-1504412 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT86312 WP_003156188.1 NZ_CP023859:1503775-1504056 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT86312 NZ_CP023859:1503775-1504056 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5