Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 116274..116875 Replicon plasmid p2_0.1
Accession NZ_CP023854
Organism Escherichia coli strain 2/0

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag CRT29_RS26590 Protein ID WP_001216034.1
Coordinates 116274..116654 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag CRT29_RS26595 Protein ID WP_001190712.1
Coordinates 116654..116875 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRT29_RS26545 111615..111922 + 308 Protein_138 type II toxin-antitoxin system ParD family antitoxin -
CRT29_RS26555 112265..112447 + 183 WP_042065278.1 hypothetical protein -
CRT29_RS26560 112568..113308 + 741 WP_001066942.1 tyrosine-type recombinase/integrase -
CRT29_RS26565 113593..114570 - 978 WP_000361610.1 RepB family plasmid replication initiator protein -
CRT29_RS26575 115366..115779 - 414 Protein_142 DDE-type integrase/transposase/recombinase -
CRT29_RS26580 115703..116062 - 360 WP_001513660.1 hypothetical protein -
CRT29_RS26585 116090..116269 - 180 WP_001513661.1 hypothetical protein -
CRT29_RS26590 116274..116654 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
CRT29_RS26595 116654..116875 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CRT29_RS26600 117058..118614 + 1557 WP_001553856.1 type I restriction-modification system subunit M -
CRT29_RS26605 118611..119882 + 1272 WP_023142242.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) / blaTEM-1B / tet(B) - 1..135053 135053
- flank IS/Tn - - 115366..115740 374


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T86310 WP_001216034.1 NZ_CP023854:c116654-116274 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T86310 NZ_CP023854:c116654-116274 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTAGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT86310 WP_001190712.1 NZ_CP023854:c116875-116654 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT86310 NZ_CP023854:c116875-116654 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References