86309

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 108799..109358 Replicon plasmid p2_0.1
Accession NZ_CP023854
Organism Escherichia coli strain 2/0

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag CRT29_RS26520 Protein ID WP_000421272.1
Coordinates 109083..109358 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag CRT29_RS26515 Protein ID WP_001387467.1
Coordinates 108799..109083 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRT29_RS26485 104262..104702 - 441 Protein_127 zinc-binding dehydrogenase -
CRT29_RS26490 104676..104870 + 195 WP_000796505.1 hypothetical protein -
CRT29_RS26495 105103..105993 + 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
CRT29_RS26500 106018..106398 + 381 WP_000710783.1 Rid family detoxifying hydrolase -
CRT29_RS26505 106431..107396 + 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
CRT29_RS26510 107442..108194 - 753 WP_000562172.1 IclR family transcriptional regulator -
CRT29_RS26515 108799..109083 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
CRT29_RS26520 109083..109358 + 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CRT29_RS26525 109464..109757 + 294 WP_001105060.1 hypothetical protein -
CRT29_RS26530 109792..109920 - 129 Protein_136 IS481 family transposase -
CRT29_RS26535 109953..111122 - 1170 WP_001072355.1 hypothetical protein -
CRT29_RS26545 111615..111922 + 308 Protein_138 type II toxin-antitoxin system ParD family antitoxin -
CRT29_RS26555 112265..112447 + 183 WP_042065278.1 hypothetical protein -
CRT29_RS26560 112568..113308 + 741 WP_001066942.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) / blaTEM-1B / tet(B) - 1..135053 135053


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T86309 WP_000421272.1 NZ_CP023854:109083-109358 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T86309 NZ_CP023854:109083-109358 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCACCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT86309 WP_001387467.1 NZ_CP023854:108799-109083 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT86309 NZ_CP023854:108799-109083 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References