Detailed information of TA system TA086294

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4242618..4243453
• Replicon: chromosome
• Accession: NZ_CP023853
• Organism: Escherichia coli strain 2/0

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A376WVB1
• Locus tag: CRT29_RS21220
• Protein ID: WP_001094448.1
• Coordinates: 4243076..4243453 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3T7EC32
• Locus tag: CRT29_RS21215
• Protein ID: WP_024175935.1
• Coordinates: 4242618..4242986 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT29_RS21190	4240047..4240280	+	234	WP_001117568.1	DUF905 family protein	-
CRT29_RS21195	4240371..4241189	+	819	WP_001234753.1	DUF945 domain-containing protein	-
CRT29_RS21200	4241281..4241766	+	486	WP_000214415.1	antirestriction protein	-
CRT29_RS21205	4241778..4242254	+	477	WP_124811586.1	RadC family protein	-
CRT29_RS21210	4242323..4242544	+	222	WP_000692346.1	DUF987 domain-containing protein	-
CRT29_RS21215	4242618..4242986	+	369	WP_024175935.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CRT29_RS21220	4243076..4243453	+	378	WP_001094448.1	TA system toxin CbtA family protein	Toxin
CRT29_RS21225	4243450..4243938	+	489	WP_000761714.1	hypothetical protein	-
CRT29_RS21230	4243950..4244171	+	222	WP_001296561.1	DUF957 domain-containing protein	-
CRT29_RS21235	4244227..4245072	+	846	WP_000065751.1	DUF4942 domain-containing protein	-
CRT29_RS21240	4245665..4246162	+	498	WP_000509815.1	hypothetical protein	-
CRT29_RS21245	4246340..4247263	+	924	WP_000535950.1	carboxylate/amino acid/amine transporter	-
CRT29_RS21250	4247267..4248085	-	819	WP_000779409.1	lipoprotein NlpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14221.22 Da
• Isoelectric Point: 8.2919

>T86294 WP_001094448.1 NZ_CP023853:4243076-4243453 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T86294 NZ_CP023853:4243076-4243453 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTACGAACTGACCAGCCAGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13598.40 Da
• Isoelectric Point: 6.6255

>AT86294 WP_024175935.1 NZ_CP023853:4242618-4242986 [Escherichia coli]
VSDTLSGTTHPDDNDDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT86294 NZ_CP023853:4242618-4242986 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A376WVB1

Antitoxin

• AlphaFold DB: A0A3T7EC32