Detailed information of TA system TA086290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 3415787..3417068
• Replicon: chromosome
• Accession: NZ_CP023853
• Organism: Escherichia coli strain 2/0

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: CRT29_RS17190
• Protein ID: WP_021528665.1
• Coordinates: 3416175..3417068 (+)
• Length: 298 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: S1P7P2
• Locus tag: CRT29_RS17185
• Protein ID: WP_000208382.1
• Coordinates: 3415787..3416182 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT29_RS17140	3411281..3411766	+	486	WP_000849565.1	antirestriction protein	-
CRT29_RS17145	3411782..3412258	+	477	WP_001186726.1	RadC family protein	-
CRT29_RS17150	3412321..3412542	+	222	WP_000692329.1	DUF987 domain-containing protein	-
CRT29_RS17155	3412561..3413205	+	645	WP_000094916.1	hypothetical protein	-
CRT29_RS17160	3413255..3413623	+	369	WP_001305076.1	type IV toxin-antitoxin system YeeU family antitoxin	-
CRT29_RS17165	3413712..3414089	+	378	WP_000854690.1	TA system toxin CbtA family protein	-
CRT29_RS17170	3414086..3414574	+	489	WP_000761699.1	hypothetical protein	-
CRT29_RS17175	3414591..3414788	+	198	WP_000839293.1	DUF957 domain-containing protein	-
CRT29_RS17180	3414873..3415718	+	846	WP_001529401.1	DUF4942 domain-containing protein	-
CRT29_RS17185	3415787..3416182	+	396	WP_000208382.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
CRT29_RS17190	3416175..3417068	+	894	WP_021528665.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
CRT29_RS17195	3417539..3417709	+	171	Protein_3296	IS110 family transposase	-
CRT29_RS17200	3418058..3419557	+	1500	WP_000174401.1	IS21-like element ISEc10 family transposase	-
CRT29_RS17205	3419554..3420309	+	756	Protein_3298	IS21-like element ISEc10 family helper ATPase IstB	-
CRT29_RS17210	3420935..3421918	+	984	WP_001296394.1	KpsF/GutQ family sugar-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (34-292)

Antitoxin

• Protein of unknown function DUF6088 (10-129)

Sequences

Toxin

Protein

• Length: 298 a.a.
• Molecular weight: 34268.37 Da
• Isoelectric Point: 5.9850

>T86290 WP_021528665.1 NZ_CP023853:3416175-3417068 [Escherichia coli]
MPELTSLFPDVADALDIESVAIVEKDYFVVDLLRLLKEIKPETHTLVFAGGTALSKAGISLNRMSEDIDIKLVPTENFMQ
NGRDKRRKIRKEIVQIITDVIHNSDIFSLDNENARITRDEYRYNEISVRYPQTFAQVPCLRPFIKLELMESTLLEHPESR
DIYSLVTELTGKGTPITAFPCATILSTQAEKLISMMRRTAAHLRNPEQQDDEFLVRHIYDNYCIVREKGVNVPVLKNFVQ
ICIQQDIERYGNQYPQFCSSPVDELKKGLDALRDYPVHKLRFQRFVNPMVFGNTQIN

Nucleotide

• Length: 894 bp

>T86290 NZ_CP023853:3416175-3417068 [Escherichia coli]
ATGCCTGAATTAACATCATTATTTCCTGACGTTGCCGATGCACTGGATATTGAATCTGTCGCTATCGTCGAAAAAGATTA
TTTTGTGGTTGATCTTTTAAGATTATTAAAAGAAATAAAACCAGAAACACATACTCTCGTGTTTGCAGGAGGTACCGCAT
TATCAAAAGCAGGTATCTCGCTAAACAGAATGTCAGAAGATATTGATATAAAGCTCGTGCCAACAGAGAACTTTATGCAA
AATGGCAGAGACAAGAGAAGAAAAATTCGCAAGGAAATCGTGCAAATAATCACGGATGTTATTCATAATTCTGACATTTT
CAGCTTGGATAATGAAAACGCCCGTATAACACGTGATGAGTATCGTTATAACGAAATCTCGGTACGTTATCCCCAGACAT
TTGCACAAGTCCCCTGTCTTCGTCCATTTATTAAGCTGGAGTTGATGGAAAGCACTCTGCTGGAACATCCGGAAAGCAGA
GATATTTATTCCCTTGTAACGGAGTTAACGGGTAAAGGTACACCTATAACAGCATTCCCCTGTGCCACCATTTTATCCAC
TCAGGCTGAAAAGCTCATCTCCATGATGCGAAGAACAGCAGCACATCTTCGTAATCCCGAACAACAGGATGATGAGTTTC
TGGTACGTCACATTTATGACAACTACTGCATTGTCAGGGAAAAAGGTGTGAATGTTCCTGTATTGAAAAATTTTGTTCAG
ATATGTATTCAACAGGATATTGAACGCTATGGTAACCAGTACCCACAATTTTGTAGCTCACCAGTTGATGAACTTAAGAA
AGGGCTGGATGCACTCCGGGATTATCCTGTACACAAATTGCGCTTTCAACGATTTGTTAACCCGATGGTATTTGGTAATA
CACAGATTAACTAG

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14640.09 Da
• Isoelectric Point: 11.3150

>AT86290 WP_000208382.1 NZ_CP023853:3415787-3416182 [Escherichia coli]
MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEGKLMKVGYGVYTKARRNIITGKIMPAAPGGSAAVITE
ALERLNIRYRLAGATECYNKGQSTQIPVSLGIKTPSRFKRMLIVGKSKINA

Nucleotide

• Length: 396 bp

>AT86290 NZ_CP023853:3415787-3416182 [Escherichia coli]
ATGACTACAAAAGACCGTATTCAGTCCCGACTGAACCGTTCAAAACGTTATGTGTTCACCCGGGATGATTTCAGGGATAT
CGCTGGCTATGATCAGGTTGGCAGAGCTCTTCGTACACTGGTAAACGAAGGTAAGTTGATGAAAGTGGGCTATGGTGTTT
ACACCAAAGCCCGTCGTAACATCATCACTGGGAAAATCATGCCCGCAGCGCCTGGGGGCTCTGCTGCAGTAATTACAGAG
GCCCTGGAGCGCCTGAATATCAGATACCGTCTGGCAGGAGCAACGGAATGCTACAATAAAGGTCAGTCCACACAAATTCC
TGTATCTCTGGGGATAAAAACACCTTCTCGATTTAAAAGAATGTTAATAGTAGGGAAAAGTAAAATCAATGCCTGA

Structures

Antitoxin

• AlphaFold DB: S1P7P2