Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2295184..2296015 Replicon chromosome
Accession NZ_CP023853
Organism Escherichia coli strain 2/0

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag CRT29_RS11810 Protein ID WP_000854815.1
Coordinates 2295641..2296015 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag CRT29_RS11805 Protein ID WP_001280918.1
Coordinates 2295184..2295552 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRT29_RS11750 2290273..2291019 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
CRT29_RS11760 2291102..2291452 + 351 Protein_2264 hypothetical protein -
CRT29_RS11765 2291468..2291878 + 411 WP_000846703.1 hypothetical protein -
CRT29_RS11775 2292099..2292917 + 819 WP_001542275.1 DUF945 domain-containing protein -
CRT29_RS11780 2292917..2293162 + 246 WP_001164966.1 hypothetical protein -
CRT29_RS11785 2293256..2293729 + 474 WP_001542276.1 antirestriction protein -
CRT29_RS11790 2293745..2294221 + 477 WP_001186200.1 RadC family protein -
CRT29_RS11795 2294284..2294505 + 222 WP_000692345.1 DUF987 domain-containing protein -
CRT29_RS11800 2294524..2295168 + 645 WP_000086752.1 hypothetical protein -
CRT29_RS11805 2295184..2295552 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CRT29_RS11810 2295641..2296015 + 375 WP_000854815.1 type IV toxin-antitoxin system toxin CbtA Toxin
CRT29_RS11815 2296012..2296206 + 195 WP_000988600.1 hypothetical protein -
CRT29_RS11820 2296219..2296332 + 114 WP_001161660.1 DUF957 domain-containing protein -
CRT29_RS11825 2296621..2296767 - 147 Protein_2276 transposase domain-containing protein -
CRT29_RS11830 2296821..2297003 + 183 WP_001296208.1 hypothetical protein -
CRT29_RS11835 2297104..2297433 - 330 WP_000450409.1 DUF496 family protein -
CRT29_RS11840 2297605..2298663 - 1059 WP_001200889.1 FUSC family protein -
CRT29_RS11845 2298861..2299334 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
CRT29_RS11850 2299453..2300619 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T86286 WP_000854815.1 NZ_CP023853:2295641-2296015 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T86286 NZ_CP023853:2295641-2296015 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT86286 WP_001280918.1 NZ_CP023853:2295184-2295552 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT86286 NZ_CP023853:2295184-2295552 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References