86268

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 33639..34437 Replicon plasmid p4_0.2
Accession NZ_CP023851
Organism Escherichia coli strain 4/0

Toxin (Protein)


Gene name ataT Uniprot ID A0A0K4UCL9
Locus tag CRT34_RS27345 Protein ID WP_024173399.1
Coordinates 33916..34437 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A0V9R6Q7
Locus tag CRT34_RS27340 Protein ID WP_001351987.1
Coordinates 33639..33908 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRT34_RS27315 29066..30406 - 1341 WP_077136336.1 AAA family ATPase -
CRT34_RS27320 30450..31190 - 741 WP_001717320.1 hypothetical protein -
CRT34_RS27325 31473..32240 + 768 WP_000342417.1 hypothetical protein -
CRT34_RS27330 32293..32646 - 354 WP_160378290.1 hypothetical protein -
CRT34_RS27335 32652..33320 - 669 WP_000161228.1 AAA family ATPase -
CRT34_RS27340 33639..33908 + 270 WP_001351987.1 DUF1778 domain-containing protein Antitoxin
CRT34_RS27345 33916..34437 + 522 WP_024173399.1 GNAT family N-acetyltransferase Toxin
CRT34_RS27355 34605..34856 - 252 WP_001404395.1 hypothetical protein -
CRT34_RS27360 34858..35550 - 693 WP_000856758.1 membrane protein -
CRT34_RS27365 35564..35887 - 324 WP_000064175.1 hypothetical protein -
CRT34_RS27375 36210..36818 - 609 WP_000274392.1 hypothetical protein -
CRT34_RS27380 36818..37072 - 255 WP_000120169.1 hypothetical protein -
CRT34_RS27385 37133..39202 - 2070 WP_053893876.1 tail fiber domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..110452 110452


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19475.31 Da        Isoelectric Point: 8.6344

>T86268 WP_024173399.1 NZ_CP023851:33916-34437 [Escherichia coli]
VDNIKIEIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEGRPKVLGYYTLSGSCFEKESLPSRSQQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLDFIQLVGNNERSL
FYPTKSIEKLFEE

Download         Length: 522 bp

>T86268 NZ_CP023851:33916-34437 [Escherichia coli]
GTGGATAATATAAAGATAGAAATTTTCTCTGGAGAGAAAGATTATGATTTGAATGGTTTTGATTGCGGTGAAGAATCACT
AAATGCCTTTTTAACCAACCACTTAAAAAGGCAGCATGAGGGAAAAATTCTTCGCGCTTATGTGCTTTGCACGAAAGAAG
GCAGACCAAAAGTGTTAGGGTATTACACTTTGTCAGGTAGCTGCTTTGAAAAGGAGTCCTTACCTTCAAGAAGCCAGCAA
AAGAAGGTACCTTATCGGAATGTTCCAAGTGTTACTTTGGGTAGGCTGGCTTTGGACAAGTCCCTTCAAGGTCAAGGATT
TGGCTCAATGCTTGTAACACATGCAATGCGCGTTGTGTACAATGCATCTCTTGCTGTAGGCATTCATGGACTTTTCGTTG
AGGCGTTAAATGACAAAGCCAAGGCGTTTTATAAAAGTTTAGACTTTATCCAGCTGGTTGGTAACAACGAGCGTTCTTTG
TTCTATCCTACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10082.54 Da        Isoelectric Point: 7.0155

>AT86268 WP_001351987.1 NZ_CP023851:33639-33908 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Download         Length: 270 bp

>AT86268 NZ_CP023851:33639-33908 [Escherichia coli]
ATGTCAGCACTAAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACTCAGTTTATGGTTAGCAGTGCATCTGAACGTGCCGTGAAAGTTATAGAGCAACACCGTAGAC
TGGTTCTGAATGAAGAATCCTGGAATCTGGTTATGGACGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCTGATCGTCTGAAAAGCATGGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4UCL9

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9R6Q7

References