86267

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 77702..78261 Replicon plasmid p4_0.1
Accession NZ_CP023850
Organism Escherichia coli strain 4/0

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag CRT34_RS26745 Protein ID WP_000421272.1
Coordinates 77702..77977 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag CRT34_RS26750 Protein ID WP_001387467.1
Coordinates 77977..78261 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRT34_RS26705 72876..73898 + 1023 WP_000361402.1 helicase UvrD -
CRT34_RS26710 74152..74849 - 698 Protein_85 IS1 family transposase -
CRT34_RS26720 75138..75445 - 308 Protein_86 type II toxin-antitoxin system ParD family antitoxin -
CRT34_RS26730 75938..77107 + 1170 WP_001072355.1 hypothetical protein -
CRT34_RS26735 77140..77268 + 129 Protein_88 IS481 family transposase -
CRT34_RS26740 77303..77596 - 294 WP_001105060.1 hypothetical protein -
CRT34_RS26745 77702..77977 - 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CRT34_RS26750 77977..78261 - 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
CRT34_RS26755 78866..79618 + 753 WP_000562172.1 IclR family transcriptional regulator -
CRT34_RS26760 79664..80629 - 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
CRT34_RS26765 80662..81042 - 381 WP_000710783.1 Rid family detoxifying hydrolase -
CRT34_RS26770 81067..81957 - 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
CRT34_RS26780 82358..82798 + 441 Protein_96 zinc-binding dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) / aac(3)-IIa / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A) - 1..138672 138672
- flank IS/Tn - - 74152..74655 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T86267 WP_000421272.1 NZ_CP023850:c77977-77702 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T86267 NZ_CP023850:c77977-77702 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT86267 WP_001387467.1 NZ_CP023850:c78261-77977 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT86267 NZ_CP023850:c78261-77977 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References