86264

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 58737..59262 Replicon plasmid p4_0.1
Accession NZ_CP023850
Organism Escherichia coli strain 4/0

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag CRT34_RS26630 Protein ID WP_001159868.1
Coordinates 58737..59042 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag CRT34_RS26635 Protein ID WP_000813634.1
Coordinates 59044..59262 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRT34_RS26615 54647..55813 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
CRT34_RS26620 56401..57156 - 756 WP_000852146.1 replication initiation protein RepE -
CRT34_RS26625 57930..58736 - 807 WP_000016982.1 site-specific integrase -
CRT34_RS26630 58737..59042 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
CRT34_RS26635 59044..59262 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CRT34_RS26640 59808..60320 + 513 WP_000151784.1 hypothetical protein -
CRT34_RS26645 60354..61505 - 1152 WP_031325939.1 DUF3800 domain-containing protein -
CRT34_RS26650 61567..61992 + 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -
CRT34_RS26655 61989..62339 + 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
CRT34_RS26660 62370..63983 + 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) / aac(3)-IIa / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A) - 1..138672 138672


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T86264 WP_001159868.1 NZ_CP023850:c59042-58737 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T86264 NZ_CP023850:c59042-58737 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT86264 WP_000813634.1 NZ_CP023850:c59262-59044 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT86264 NZ_CP023850:c59262-59044 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References