Detailed information of TA system TA086258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 5139723..5140543
• Replicon: chromosome
• Accession: NZ_CP023849
• Organism: Escherichia coli strain 4/0

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: CRT34_RS25690
• Protein ID: WP_001054379.1
• Coordinates: 5140286..5140543 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: CRT34_RS25685
• Protein ID: WP_000123957.1
• Coordinates: 5139723..5140274 (-)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT34_RS25660	5135417..5136730	-	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-
CRT34_RS25665	5136742..5137020	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
CRT34_RS25670	5137017..5138138	-	1122	WP_000010833.1	M42 family metallopeptidase	-
CRT34_RS28180	5138383..5138499	-	117	Protein_4911	VOC family protein	-
CRT34_RS25675	5138537..5138797	-	261	WP_000077645.1	hypothetical protein	-
CRT34_RS25680	5138925..5139671	-	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
CRT34_RS25685	5139723..5140274	-	552	WP_000123957.1	N-acetyltransferase	Antitoxin
CRT34_RS25690	5140286..5140543	-	258	WP_001054379.1	YjhX family toxin	Toxin
CRT34_RS25695	5140920..5141084	-	165	Protein_4916	GNAT family N-acetyltransferase	-
CRT34_RS25705	5142327..5143433	+	1107	Protein_4918	helicase YjhR	-
CRT34_RS25710	5144016..5144996	-	981	WP_000991462.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T86258 WP_001054379.1 NZ_CP023849:c5140543-5140286 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T86258 NZ_CP023849:c5140543-5140286 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT86258 WP_000123957.1 NZ_CP023849:c5140274-5139723 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT86258 NZ_CP023849:c5140274-5139723 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030