Detailed information of TA system TA086257

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5104500..5105278
• Replicon: chromosome
• Accession: NZ_CP023849
• Organism: Escherichia coli strain 4/0

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: CRT34_RS25515
• Protein ID: WP_124809540.1
• Coordinates: 5104958..5105278 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: CRT34_RS25510
• Protein ID: WP_001295723.1
• Coordinates: 5104500..5104868 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT34_RS25475	5101615..5101773	+	159	WP_001323397.1	DUF905 family protein	-
CRT34_RS25485	5101928..5102746	+	819	WP_001234738.1	DUF945 domain-containing protein	-
CRT34_RS25495	5103088..5103561	+	474	WP_001350782.1	antirestriction protein	-
CRT34_RS25500	5103577..5104053	+	477	WP_001186775.1	RadC family protein	-
CRT34_RS25505	5104116..5104337	+	222	WP_000692345.1	DUF987 domain-containing protein	-
CRT34_RS25510	5104500..5104868	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CRT34_RS25515	5104958..5105278	+	321	WP_124809540.1	TA system toxin CbtA family protein	Toxin
CRT34_RS25520	5105332..5106078	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
CRT34_RS25525	5106093..5107634	-	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
CRT34_RS28035	5107829..5107921	+	93	Protein_4882	toxin	-
CRT34_RS25530	5107918..5108406	+	489	WP_000761690.1	hypothetical protein	-
CRT34_RS25535	5108423..5108599	+	177	WP_000839286.1	DUF957 domain-containing protein	-
CRT34_RS28040	5108699..5108857	+	159	WP_001467148.1	hypothetical protein	-
CRT34_RS25545	5109221..5109397	+	177	Protein_4886	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-99)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11934.74 Da
• Isoelectric Point: 5.0717

>T86257 WP_124809540.1 NZ_CP023849:5104958-5105278 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRVRITVKEIGE

Nucleotide

• Length: 321 bp

>T86257 NZ_CP023849:5104958-5105278 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGTGCGTATTACCGTGAAGGAGATCGGTGAGTA
A

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT86257 WP_001295723.1 NZ_CP023849:5104500-5104868 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT86257 NZ_CP023849:5104500-5104868 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: S1NM52