Detailed information of TA system TA086249

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3430047..3430881
• Replicon: chromosome
• Accession: NZ_CP023849
• Organism: Escherichia coli strain 4/0

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: CRT34_RS17345
• Protein ID: WP_000854690.1
• Coordinates: 3430504..3430881 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1P7N8
• Locus tag: CRT34_RS17340
• Protein ID: WP_001305076.1
• Coordinates: 3430047..3430415 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT34_RS17295	3425129..3426256	+	1128	Protein_3321	hypothetical protein	-
CRT34_RS17300	3426332..3426787	+	456	WP_000581502.1	hypothetical protein	-
CRT34_RS17305	3426866..3427099	+	234	WP_000902034.1	DUF905 family protein	-
CRT34_RS17315	3427200..3428018	+	819	WP_001234620.1	DUF945 domain-containing protein	-
CRT34_RS17320	3428073..3428558	+	486	WP_000849565.1	antirestriction protein	-
CRT34_RS17325	3428574..3429050	+	477	WP_001186726.1	RadC family protein	-
CRT34_RS17330	3429113..3429334	+	222	WP_000692329.1	DUF987 domain-containing protein	-
CRT34_RS17335	3429353..3429997	+	645	WP_000094916.1	hypothetical protein	-
CRT34_RS17340	3430047..3430415	+	369	WP_001305076.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CRT34_RS17345	3430504..3430881	+	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
CRT34_RS17350	3430878..3431366	+	489	WP_000761699.1	hypothetical protein	-
CRT34_RS17355	3431383..3431580	+	198	WP_000839293.1	DUF957 domain-containing protein	-
CRT34_RS17360	3431665..3432510	+	846	WP_001529401.1	DUF4942 domain-containing protein	-
CRT34_RS17365	3432579..3432974	+	396	WP_000208384.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
CRT34_RS17370	3432967..3433900	+	934	Protein_3335	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
CRT34_RS28000	3434332..3434487	+	156	WP_000729638.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3434332..3434487	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T86249 WP_000854690.1 NZ_CP023849:3430504-3430881 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T86249 NZ_CP023849:3430504-3430881 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13560.39 Da
• Isoelectric Point: 4.7830

>AT86249 WP_001305076.1 NZ_CP023849:3430047-3430415 [Escherichia coli]
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT86249 NZ_CP023849:3430047-3430415 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCTGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: S1PLF5

Antitoxin

• AlphaFold DB: S1P7N8