Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2272337..2273168 Replicon chromosome
Accession NZ_CP023849
Organism Escherichia coli strain 4/0

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag CRT34_RS11735 Protein ID WP_000854815.1
Coordinates 2272794..2273168 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag CRT34_RS11730 Protein ID WP_001280918.1
Coordinates 2272337..2272705 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRT34_RS11675 2267426..2268172 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
CRT34_RS11685 2268255..2268605 + 351 Protein_2251 hypothetical protein -
CRT34_RS11690 2268621..2269031 + 411 WP_000846703.1 hypothetical protein -
CRT34_RS11700 2269252..2270070 + 819 WP_001542275.1 DUF945 domain-containing protein -
CRT34_RS11705 2270070..2270315 + 246 WP_001164966.1 hypothetical protein -
CRT34_RS11710 2270409..2270882 + 474 WP_001542276.1 antirestriction protein -
CRT34_RS11715 2270898..2271374 + 477 WP_001186200.1 RadC family protein -
CRT34_RS11720 2271437..2271658 + 222 WP_000692345.1 DUF987 domain-containing protein -
CRT34_RS11725 2271677..2272321 + 645 WP_000086752.1 hypothetical protein -
CRT34_RS11730 2272337..2272705 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CRT34_RS11735 2272794..2273168 + 375 WP_000854815.1 type IV toxin-antitoxin system toxin CbtA Toxin
CRT34_RS11740 2273165..2273359 + 195 WP_000988600.1 hypothetical protein -
CRT34_RS11745 2273372..2273485 + 114 WP_001161660.1 DUF957 domain-containing protein -
CRT34_RS11750 2273774..2273920 - 147 Protein_2263 transposase domain-containing protein -
CRT34_RS11755 2273974..2274156 + 183 WP_001296208.1 hypothetical protein -
CRT34_RS11760 2274257..2274586 - 330 WP_000450409.1 DUF496 family protein -
CRT34_RS11765 2274758..2275816 - 1059 WP_001200889.1 FUSC family protein -
CRT34_RS11770 2276014..2276487 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
CRT34_RS11775 2276606..2277772 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T86246 WP_000854815.1 NZ_CP023849:2272794-2273168 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T86246 NZ_CP023849:2272794-2273168 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT86246 WP_001280918.1 NZ_CP023849:2272337-2272705 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT86246 NZ_CP023849:2272337-2272705 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References