Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 113595..114120 Replicon plasmid p4_1_1.1
Accession NZ_CP023845
Organism Escherichia coli strain 4/1-1

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag CRT35_RS26395 Protein ID WP_001159868.1
Coordinates 113815..114120 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag CRT35_RS26390 Protein ID WP_000813634.1
Coordinates 113595..113813 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRT35_RS26375 111330..112463 + 1134 WP_000545983.1 DUF3800 domain-containing protein -
CRT35_RS26380 112483..112767 + 285 WP_000642771.1 hypothetical protein -
CRT35_RS26385 112764..112961 - 198 WP_000215657.1 hypothetical protein -
CRT35_RS26390 113595..113813 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CRT35_RS26395 113815..114120 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
CRT35_RS26400 114121..114927 + 807 WP_000016982.1 site-specific integrase -
CRT35_RS26405 115701..116456 + 756 WP_000852146.1 replication initiation protein RepE -
CRT35_RS26410 117044..118210 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / blaTEM-1B / aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 - 1..181324 181324


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T86223 WP_001159868.1 NZ_CP023845:113815-114120 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T86223 NZ_CP023845:113815-114120 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT86223 WP_000813634.1 NZ_CP023845:113595-113813 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT86223 NZ_CP023845:113595-113813 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References