Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 94408..95009 Replicon plasmid p4_1_1.1
Accession NZ_CP023845
Organism Escherichia coli strain 4/1-1

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag CRT35_RS26310 Protein ID WP_001216034.1
Coordinates 94408..94788 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag CRT35_RS26315 Protein ID WP_001190712.1
Coordinates 94788..95009 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRT35_RS26275 89459..90391 - 933 WP_000081352.1 homocysteine S-methyltransferase -
CRT35_RS26280 90378..91781 - 1404 WP_001373486.1 S-methylmethionine permease -
CRT35_RS26285 91989..93005 + 1017 WP_012372828.1 IS5-like element IS5 family transposase -
CRT35_RS26290 93233..93550 + 318 WP_001513659.1 hypothetical protein -
CRT35_RS26295 93500..93913 - 414 Protein_112 DDE-type integrase/transposase/recombinase -
CRT35_RS26300 93837..94196 - 360 WP_001513660.1 hypothetical protein -
CRT35_RS26305 94224..94403 - 180 WP_001513661.1 hypothetical protein -
CRT35_RS26310 94408..94788 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
CRT35_RS26315 94788..95009 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CRT35_RS26320 95192..96748 + 1557 WP_001553856.1 type I restriction-modification system subunit M -
CRT35_RS26325 96745..98016 + 1272 WP_048266692.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / blaTEM-1B / aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 - 1..181324 181324


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T86221 WP_001216034.1 NZ_CP023845:c94788-94408 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T86221 NZ_CP023845:c94788-94408 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTAGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT86221 WP_001190712.1 NZ_CP023845:c95009-94788 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT86221 NZ_CP023845:c95009-94788 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References