Detailed information of TA system TA086220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 80534..81093
• Replicon: plasmid p4_1_1.1
• Accession: NZ_CP023845
• Organism: Escherichia coli strain 4/1-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0SVC8
• Locus tag: CRT35_RS26205
• Protein ID: WP_000421272.1
• Coordinates: 80818..81093 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: CRT35_RS26200
• Protein ID: WP_001387467.1
• Coordinates: 80534..80818 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT35_RS26170	75997..76437	-	441	Protein_90	zinc-binding dehydrogenase	-
CRT35_RS26175	76411..76605	+	195	WP_000796505.1	hypothetical protein	-
CRT35_RS26180	76838..77728	+	891	WP_001077068.1	dihydrodipicolinate synthase family protein	-
CRT35_RS26185	77753..78133	+	381	WP_000710783.1	Rid family detoxifying hydrolase	-
CRT35_RS26190	78166..79131	+	966	WP_001022265.1	2-keto-3-deoxygluconate transporter	-
CRT35_RS26195	79177..79929	-	753	WP_000562172.1	IclR family transcriptional regulator	-
CRT35_RS26200	80534..80818	+	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
CRT35_RS26205	80818..81093	+	276	WP_000421272.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CRT35_RS26210	81199..81492	+	294	WP_001105060.1	hypothetical protein	-
CRT35_RS26215	81527..81655	-	129	Protein_99	IS481 family transposase	-
CRT35_RS26220	81688..82857	-	1170	WP_001072355.1	hypothetical protein	-
CRT35_RS26230	83350..83657	+	308	Protein_101	type II toxin-antitoxin system ParD family antitoxin	-
CRT35_RS26240	84000..84182	+	183	WP_042065278.1	hypothetical protein	-
CRT35_RS26245	84303..85043	+	741	WP_001066942.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / blaTEM-1B / aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12	-	1..181324	181324

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 10.0871

>T86220 WP_000421272.1 NZ_CP023845:80818-81093 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T86220 NZ_CP023845:80818-81093 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT86220 WP_001387467.1 NZ_CP023845:80534-80818 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT86220 NZ_CP023845:80534-80818 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: V0SVC8

Antitoxin

• AlphaFold DB: A0A0E0Y880