Detailed information of TA system TA086218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4995470..4996290
• Replicon: chromosome
• Accession: NZ_CP023844
• Organism: Escherichia coli strain 4/1-1

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: CRT35_RS24865
• Protein ID: WP_001054379.1
• Coordinates: 4996033..4996290 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: CRT35_RS24860
• Protein ID: WP_000123957.1
• Coordinates: 4995470..4996021 (-)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT35_RS24835	4991164..4992477	-	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-
CRT35_RS24840	4992489..4992767	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
CRT35_RS24845	4992764..4993885	-	1122	WP_000010833.1	M42 family metallopeptidase	-
CRT35_RS27440	4994130..4994246	-	117	Protein_4739	VOC family protein	-
CRT35_RS24850	4994284..4994544	-	261	WP_000077645.1	hypothetical protein	-
CRT35_RS24855	4994672..4995418	-	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
CRT35_RS24860	4995470..4996021	-	552	WP_000123957.1	N-acetyltransferase	Antitoxin
CRT35_RS24865	4996033..4996290	-	258	WP_001054379.1	YjhX family toxin	Toxin
CRT35_RS24870	4996667..4996831	-	165	Protein_4744	GNAT family N-acetyltransferase	-
CRT35_RS24880	4998074..4999180	+	1107	Protein_4746	helicase YjhR	-
CRT35_RS24885	4999763..5000743	-	981	WP_000991462.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T86218 WP_001054379.1 NZ_CP023844:c4996290-4996033 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T86218 NZ_CP023844:c4996290-4996033 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT86218 WP_000123957.1 NZ_CP023844:c4996021-4995470 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT86218 NZ_CP023844:c4996021-4995470 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030