Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3949031..3949831 Replicon chromosome
Accession NZ_CP023844
Organism Escherichia coli strain 4/1-1

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag CRT35_RS19830 Protein ID WP_000342452.1
Coordinates 3949031..3949558 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag CRT35_RS19835 Protein ID WP_001277107.1
Coordinates 3949565..3949831 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRT35_RS19805 3944107..3944874 - 768 WP_000082099.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
CRT35_RS19810 3944871..3946148 - 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
CRT35_RS19815 3946145..3947071 - 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
CRT35_RS19820 3947119..3948228 - 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
CRT35_RS19825 3948651..3949034 + 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
CRT35_RS19830 3949031..3949558 - 528 WP_000342452.1 GNAT family N-acetyltransferase Toxin
CRT35_RS19835 3949565..3949831 - 267 WP_001277107.1 DUF1778 domain-containing protein Antitoxin
CRT35_RS19840 3949980..3951083 - 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
CRT35_RS19845 3951354..3952208 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
CRT35_RS19850 3952453..3953511 - 1059 WP_001042013.1 permease-like cell division protein FtsX -
CRT35_RS19855 3953504..3954172 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T86213 WP_000342452.1 NZ_CP023844:c3949558-3949031 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T86213 NZ_CP023844:c3949558-3949031 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT86213 WP_001277107.1 NZ_CP023844:c3949831-3949565 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT86213 NZ_CP023844:c3949831-3949565 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References