Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2236962..2237793 Replicon chromosome
Accession NZ_CP023844
Organism Escherichia coli strain 4/1-1

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag CRT35_RS11460 Protein ID WP_000854815.1
Coordinates 2237419..2237793 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag CRT35_RS11455 Protein ID WP_001280918.1
Coordinates 2236962..2237330 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRT35_RS11400 2232051..2232797 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
CRT35_RS11410 2232880..2233230 + 351 Protein_2189 hypothetical protein -
CRT35_RS11415 2233246..2233656 + 411 WP_000846703.1 hypothetical protein -
CRT35_RS11425 2233877..2234695 + 819 WP_001542275.1 DUF945 domain-containing protein -
CRT35_RS11430 2234695..2234940 + 246 WP_001164966.1 hypothetical protein -
CRT35_RS11435 2235034..2235507 + 474 WP_001542276.1 antirestriction protein -
CRT35_RS11440 2235523..2235999 + 477 WP_001186200.1 RadC family protein -
CRT35_RS11445 2236062..2236283 + 222 WP_000692345.1 DUF987 domain-containing protein -
CRT35_RS11450 2236302..2236946 + 645 WP_000086752.1 hypothetical protein -
CRT35_RS11455 2236962..2237330 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CRT35_RS11460 2237419..2237793 + 375 WP_000854815.1 type IV toxin-antitoxin system toxin CbtA Toxin
CRT35_RS11465 2237790..2237984 + 195 WP_000988600.1 hypothetical protein -
CRT35_RS11470 2237997..2238110 + 114 WP_001161660.1 DUF957 domain-containing protein -
CRT35_RS11475 2238399..2238545 - 147 Protein_2201 transposase domain-containing protein -
CRT35_RS11480 2238599..2238781 + 183 WP_001296208.1 hypothetical protein -
CRT35_RS11485 2238882..2239211 - 330 WP_000450409.1 DUF496 family protein -
CRT35_RS11490 2239383..2240441 - 1059 WP_001200889.1 FUSC family protein -
CRT35_RS11495 2240639..2241112 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
CRT35_RS11500 2241231..2242397 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2225276..2239211 13935


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T86207 WP_000854815.1 NZ_CP023844:2237419-2237793 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T86207 NZ_CP023844:2237419-2237793 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT86207 WP_001280918.1 NZ_CP023844:2236962-2237330 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT86207 NZ_CP023844:2236962-2237330 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References