Detailed information of TA system TA086188

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 108309..108834
• Replicon: plasmid p4_1_2.1
• Accession: NZ_CP023840
• Organism: Klebsiella pneumoniae strain 4/1-2

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: CRT36_RS27510
• Protein ID: WP_012539978.1
• Coordinates: 108529..108834 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: R4WC25
• Locus tag: CRT36_RS27505
• Protein ID: WP_015632547.1
• Coordinates: 108309..108527 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT36_RS27480	103554..105056	+	1503	WP_048296350.1	RES family NAD+ phosphorylase	-
CRT36_RS27485	105249..105599	-	351	WP_015632544.1	hypothetical protein	-
CRT36_RS27490	106177..106362	+	186	WP_032433930.1	hypothetical protein	-
CRT36_RS27495	106435..107709	-	1275	WP_015632546.1	hypothetical protein	-
CRT36_RS27505	108309..108527	+	219	WP_015632547.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
CRT36_RS27510	108529..108834	+	306	WP_012539978.1	type II toxin-antitoxin system toxin CcdB	Toxin
CRT36_RS27515	109021..110025	+	1005	WP_029497484.1	hypothetical protein	-
CRT36_RS27520	110223..111002	+	780	WP_015632549.1	site-specific integrase	-
CRT36_RS27525	111060..111317	-	258	WP_032433931.1	hypothetical protein	-
CRT36_RS29255	111445..111558	-	114	WP_032072094.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..112141	112141

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11601.27 Da
• Isoelectric Point: 6.4661

>T86188 WP_012539978.1 NZ_CP023840:108529-108834 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T86188 NZ_CP023840:108529-108834 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8362.31 Da
• Isoelectric Point: 4.7247

>AT86188 WP_015632547.1 NZ_CP023840:108309-108527 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIDMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT86188 NZ_CP023840:108309-108527 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGATATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2L1KT86