Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1498158..1498894 Replicon chromosome
Accession NZ_CP023839
Organism Klebsiella pneumoniae strain 4/1-2

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag CRT36_RS07485 Protein ID WP_032433360.1
Coordinates 1498412..1498894 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CRT36_RS07480 Protein ID WP_003026799.1
Coordinates 1498158..1498424 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRT36_RS07455 1493804..1494943 + 1140 WP_032433368.1 mannitol dehydrogenase -
CRT36_RS07460 1494972..1495634 + 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
CRT36_RS07465 1495618..1496622 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
CRT36_RS07470 1496640..1497272 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
CRT36_RS07475 1497282..1497845 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
CRT36_RS07480 1498158..1498424 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CRT36_RS07485 1498412..1498894 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
CRT36_RS07490 1499052..1500584 - 1533 WP_015065592.1 IS3-like element ISKpn38 family transposase -
CRT36_RS07495 1500696..1502099 + 1404 WP_039815096.1 ISNCY-like element ISKpn21 family transposase -
CRT36_RS07500 1502128..1502760 - 633 WP_001567369.1 hypothetical protein -
CRT36_RS07505 1503140..1503739 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1466103..1513722 47619


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T86173 WP_032433360.1 NZ_CP023839:1498412-1498894 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T86173 NZ_CP023839:1498412-1498894 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT86173 WP_003026799.1 NZ_CP023839:1498158-1498424 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT86173 NZ_CP023839:1498158-1498424 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References