86168

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 13428..13893 Replicon plasmid p4_2_1.2
Accession NZ_CP023836
Organism Escherichia coli strain 4/2-1

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag CRT37_RS23915 Protein ID WP_001230707.1
Coordinates 13428..13679 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag CRT37_RS23920 Protein ID WP_000801440.1
Coordinates 13666..13893 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRT37_RS23890 8826..9692 - 867 WP_001353743.1 replication initiation protein -
CRT37_RS23895 10947..11282 + 336 WP_124759838.1 type II toxin-antitoxin system RelE/ParE family toxin -
CRT37_RS23900 11279..11629 + 351 WP_000127482.1 addiction module antitoxin -
CRT37_RS23905 12269..12784 + 516 WP_001025395.1 J domain-containing protein -
CRT37_RS23910 12889..13431 + 543 WP_001083906.1 hypothetical protein -
CRT37_RS23915 13428..13679 + 252 WP_001230707.1 hypothetical protein Toxin
CRT37_RS23920 13666..13893 + 228 WP_000801440.1 hypothetical protein Antitoxin
CRT37_RS23930 14347..14646 + 300 WP_000650311.1 hypothetical protein -
CRT37_RS23935 14656..15072 + 417 WP_012421208.1 tail-associated lysozyme -
CRT37_RS23940 15069..15380 + 312 WP_000627400.1 hypothetical protein -
CRT37_RS23945 15685..15816 + 132 WP_001353745.1 toxin-antitoxin system HicB family antitoxin -
CRT37_RS23955 16177..16680 + 504 WP_000781814.1 hypothetical protein -
CRT37_RS23960 16684..17913 + 1230 WP_000539543.1 relaxase/mobilization nuclease domain-containing protein -
CRT37_RS23965 17990..18283 + 294 WP_000134733.1 LuxR family transcriptional regulator -
CRT37_RS23970 18280..18495 + 216 WP_000760375.1 EexN family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..33067 33067


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-87)

Antitoxin

Protein of unknown function DUF6290

(1-64)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T86168 WP_001230707.1 NZ_CP023836:13428-13679 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T86168 NZ_CP023836:13428-13679 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCCGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT86168 WP_000801440.1 NZ_CP023836:13666-13893 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT86168 NZ_CP023836:13666-13893 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References