Detailed information of TA system TA086136

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 46353..46954
• Replicon: plasmid p4_4.1
• Accession: NZ_CP023827
• Organism: Escherichia coli strain 4/4

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: CRT43_RS26005
• Protein ID: WP_001216034.1
• Coordinates: 46574..46954 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: CRT43_RS26000
• Protein ID: WP_001190712.1
• Coordinates: 46353..46574 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT43_RS25970	41360..42166	-	807	WP_000016982.1	site-specific integrase	-
CRT43_RS25975	42167..42472	-	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	-
CRT43_RS25980	42474..42692	-	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	-
CRT43_RS25985	43400..44395	+	996	WP_000246636.1	hypothetical protein	-
CRT43_RS25990	44399..45331	+	933	WP_000991832.1	hypothetical protein	-
CRT43_RS25995	45468..46165	-	698	Protein_61	IS1 family transposase	-
CRT43_RS26000	46353..46574	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CRT43_RS26005	46574..46954	+	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
CRT43_RS26010	46959..47138	+	180	WP_001513661.1	hypothetical protein	-
CRT43_RS26015	47166..47525	+	360	WP_001513660.1	hypothetical protein	-
CRT43_RS26020	47449..47862	+	414	Protein_66	DDE-type integrase/transposase/recombinase	-
CRT43_RS26025	47812..48129	-	318	WP_001513659.1	hypothetical protein	-
CRT43_RS26030	48357..49337	-	981	WP_000019407.1	IS5-like element IS5 family transposase	-
CRT43_RS26035	49581..50984	+	1404	WP_001373486.1	S-methylmethionine permease	-
CRT43_RS26040	50971..51903	+	933	WP_000081352.1	homocysteine S-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27	senB	1..125877	125877
	-	inside	IScluster/Tn	-	-	45468..52860	7392

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T86136 WP_001216034.1 NZ_CP023827:46574-46954 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T86136 NZ_CP023827:46574-46954 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT86136 WP_001190712.1 NZ_CP023827:46353-46574 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT86136 NZ_CP023827:46353-46574 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: V0AJ64

Antitoxin

• AlphaFold DB: A0A829CJB6