Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 42167..42692 Replicon plasmid p4_4.1
Accession NZ_CP023827
Organism Escherichia coli strain 4/4

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag CRT43_RS25975 Protein ID WP_001159868.1
Coordinates 42167..42472 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag CRT43_RS25980 Protein ID WP_000813634.1
Coordinates 42474..42692 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRT43_RS25960 38077..39243 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
CRT43_RS25965 39831..40586 - 756 WP_000852146.1 replication initiation protein RepE -
CRT43_RS25970 41360..42166 - 807 WP_000016982.1 site-specific integrase -
CRT43_RS25975 42167..42472 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
CRT43_RS25980 42474..42692 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CRT43_RS25985 43400..44395 + 996 WP_000246636.1 hypothetical protein -
CRT43_RS25990 44399..45331 + 933 WP_000991832.1 hypothetical protein -
CRT43_RS25995 45468..46165 - 698 Protein_61 IS1 family transposase -
CRT43_RS26000 46353..46574 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
CRT43_RS26005 46574..46954 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin -
CRT43_RS26010 46959..47138 + 180 WP_001513661.1 hypothetical protein -
CRT43_RS26015 47166..47525 + 360 WP_001513660.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27 senB 1..125877 125877
- inside IScluster/Tn - - 45468..52860 7392


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T86135 WP_001159868.1 NZ_CP023827:c42472-42167 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T86135 NZ_CP023827:c42472-42167 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT86135 WP_000813634.1 NZ_CP023827:c42692-42474 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT86135 NZ_CP023827:c42692-42474 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References