86118

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2263552..2264383 Replicon chromosome
Accession NZ_CP023826
Organism Escherichia coli strain 4/4

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag CRT43_RS11715 Protein ID WP_000854815.1
Coordinates 2264009..2264383 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag CRT43_RS11710 Protein ID WP_001280918.1
Coordinates 2263552..2263920 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRT43_RS11655 2258641..2259387 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
CRT43_RS11665 2259470..2259820 + 351 Protein_2248 hypothetical protein -
CRT43_RS11670 2259836..2260246 + 411 WP_000846703.1 hypothetical protein -
CRT43_RS11680 2260467..2261285 + 819 WP_001542275.1 DUF945 domain-containing protein -
CRT43_RS11685 2261285..2261530 + 246 WP_001164966.1 hypothetical protein -
CRT43_RS11690 2261624..2262097 + 474 WP_001542276.1 antirestriction protein -
CRT43_RS11695 2262113..2262589 + 477 WP_001186200.1 RadC family protein -
CRT43_RS11700 2262652..2262873 + 222 WP_000692345.1 DUF987 domain-containing protein -
CRT43_RS11705 2262892..2263536 + 645 WP_000086752.1 hypothetical protein -
CRT43_RS11710 2263552..2263920 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CRT43_RS11715 2264009..2264383 + 375 WP_000854815.1 type IV toxin-antitoxin system toxin CbtA Toxin
CRT43_RS11720 2264380..2264574 + 195 WP_000988600.1 hypothetical protein -
CRT43_RS11725 2264587..2264700 + 114 WP_001161660.1 DUF957 domain-containing protein -
CRT43_RS11730 2264989..2265135 - 147 Protein_2260 transposase domain-containing protein -
CRT43_RS11735 2265189..2265371 + 183 WP_001296208.1 hypothetical protein -
CRT43_RS11740 2265472..2265801 - 330 WP_000450409.1 DUF496 family protein -
CRT43_RS11745 2265973..2267031 - 1059 WP_001200889.1 FUSC family protein -
CRT43_RS11750 2267229..2267702 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
CRT43_RS11755 2267821..2268987 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T86118 WP_000854815.1 NZ_CP023826:2264009-2264383 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T86118 NZ_CP023826:2264009-2264383 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT86118 WP_001280918.1 NZ_CP023826:2263552-2263920 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT86118 NZ_CP023826:2263552-2263920 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References