Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 65097..65622 Replicon plasmid p7_2.1
Accession NZ_CP023821
Organism Escherichia coli strain 7/2

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CRT46_RS25580 Protein ID WP_001159871.1
Coordinates 65097..65402 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag CRT46_RS25585 Protein ID WP_000813634.1
Coordinates 65404..65622 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRT46_RS25545 60524..61321 + 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -
CRT46_RS25550 61383..61634 + 252 Protein_76 DUF4113 domain-containing protein -
CRT46_RS25555 61639..62031 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
CRT46_RS25560 62036..63007 - 972 WP_001103694.1 hypothetical protein -
CRT46_RS25565 63236..63880 + 645 WP_000633911.1 AAA family ATPase -
CRT46_RS25570 63874..64149 + 276 WP_000239529.1 hypothetical protein -
CRT46_RS25575 64287..65096 - 810 WP_000016979.1 site-specific integrase -
CRT46_RS25580 65097..65402 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CRT46_RS25585 65404..65622 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CRT46_RS25595 67943..68584 + 642 WP_000990667.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) senB 1..113737 113737


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T86100 WP_001159871.1 NZ_CP023821:c65402-65097 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T86100 NZ_CP023821:c65402-65097 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT86100 WP_000813634.1 NZ_CP023821:c65622-65404 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT86100 NZ_CP023821:c65622-65404 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References