Detailed information of TA system TA086075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1831404..1831930
• Replicon: chromosome
• Accession: NZ_CP023820
• Organism: Escherichia coli strain 7/2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: CRT46_RS08960
• Protein ID: WP_000323025.1
• Coordinates: 1831404..1831691 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: CRT46_RS08965
• Protein ID: WP_000534858.1
• Coordinates: 1831691..1831930 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT46_RS08910	1826488..1826946	-	459	WP_000526135.1	IS200/IS605 family transposase	-
CRT46_RS08920	1827397..1827612	-	216	WP_000066484.1	cold shock-like protein CspB	-
CRT46_RS08925	1827913..1828125	+	213	WP_000087756.1	cold shock-like protein CspF	-
CRT46_RS08930	1828180..1828269	+	90	WP_120795389.1	hypothetical protein	-
CRT46_RS08935	1828547..1829299	-	753	WP_001047135.1	antitermination protein	-
CRT46_RS08940	1829313..1830362	-	1050	WP_001265199.1	DUF968 domain-containing protein	-
CRT46_RS08945	1830364..1830642	-	279	WP_012304870.1	hypothetical protein	-
CRT46_RS08950	1830709..1830960	-	252	WP_000980994.1	hypothetical protein	-
CRT46_RS08955	1831177..1831332	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
CRT46_RS08960	1831404..1831691	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
CRT46_RS08965	1831691..1831930	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
CRT46_RS08970	1831955..1832260	+	306	WP_001326990.1	hypothetical protein	-
CRT46_RS08975	1832463..1832795	+	333	WP_001301033.1	protein FlxA	-
CRT46_RS08980	1833232..1834545	-	1314	WP_000589005.1	ISNCY family transposase	-
CRT46_RS08985	1835029..1836057	-	1029	WP_001345551.1	DUF4868 domain-containing protein	-
CRT46_RS08990	1836054..1836668	-	615	WP_001265627.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1797734..1849155	51421

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T86075 WP_000323025.1 NZ_CP023820:c1831691-1831404 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T86075 NZ_CP023820:c1831691-1831404 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT86075 WP_000534858.1 NZ_CP023820:c1831930-1831691 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT86075 NZ_CP023820:c1831930-1831691 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8