Detailed information of TA system TA086066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1210229..1211013
• Replicon: chromosome
• Accession: NZ_CP023820
• Organism: Escherichia coli strain 7/2

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: CRT46_RS05675
• Protein ID: WP_000613626.1
• Coordinates: 1210229..1210723 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: CRT46_RS05680
• Protein ID: WP_001110447.1
• Coordinates: 1210720..1211013 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT46_RS05655	1205422..1206519	+	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-
CRT46_RS05660	1206519..1207460	+	942	WP_001522863.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
CRT46_RS05665	1207526..1209169	+	1644	WP_001522865.1	flagellar hook-associated protein FlgK	-
CRT46_RS05670	1209181..1210134	+	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
CRT46_RS05675	1210229..1210723	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
CRT46_RS05680	1210720..1211013	-	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
CRT46_RS05685	1211146..1214331	-	3186	WP_001522867.1	ribonuclease E	-
CRT46_RS05695	1214904..1215863	+	960	WP_000846343.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T86066 WP_000613626.1 NZ_CP023820:c1210723-1210229 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T86066 NZ_CP023820:c1210723-1210229 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT86066 WP_001110447.1 NZ_CP023820:c1211013-1210720 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT86066 NZ_CP023820:c1211013-1210720 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6