Detailed information of TA system TA086065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 920932..921637
• Replicon: chromosome
• Accession: NZ_CP023820
• Organism: Escherichia coli strain 7/2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: CRT46_RS04240
• Protein ID: WP_000539521.1
• Coordinates: 921251..921637 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CRT46_RS04235
• Protein ID: WP_001280945.1
• Coordinates: 920932..921261 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT46_RS04220	916089..917000	+	912	WP_001236019.1	glutathione ABC transporter permease GsiD	-
CRT46_RS04225	917178..919526	+	2349	WP_021523051.1	EAL domain-containing protein	-
CRT46_RS04230	919534..920862	+	1329	WP_000086873.1	GGDEF domain-containing protein	-
CRT46_RS04235	920932..921261	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
CRT46_RS04240	921251..921637	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CRT46_RS04245	921863..923188	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
CRT46_RS04250	923401..923784	+	384	WP_000497137.1	biofilm formation regulator BssR	-
CRT46_RS04255	923895..925010	+	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
CRT46_RS04260	925007..925633	-	627	WP_020233670.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T86065 WP_000539521.1 NZ_CP023820:c921637-921251 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T86065 NZ_CP023820:c921637-921251 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT86065 WP_001280945.1 NZ_CP023820:c921261-920932 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT86065 NZ_CP023820:c921261-920932 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6