Detailed information of TA system TA086061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 55041..55528
• Replicon: chromosome
• Accession: NZ_CP023820
• Organism: Escherichia coli strain 7/2

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: CRT46_RS00255
• Protein ID: WP_001520461.1
• Coordinates: 55277..55528 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A2T1MJK4
• Locus tag: CRT46_RS00250
• Protein ID: WP_001520458.1
• Coordinates: 55041..55274 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT46_RS00225	50114..50401	+	288	WP_000203747.1	ferredoxin-like protein FixX	-
CRT46_RS00230	50460..51791	+	1332	WP_001520455.1	MFS transporter	-
CRT46_RS00235	51899..52429	+	531	WP_021522993.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
CRT46_RS00240	52422..54284	+	1863	WP_000377113.1	glutathione-regulated potassium-efflux system protein KefC	-
CRT46_RS00245	54476..54955	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
CRT46_RS00250	55041..55274	+	234	WP_001520458.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
CRT46_RS00255	55277..55528	+	252	WP_001520461.1	CcdB family protein	Toxin
CRT46_RS00260	55588..56436	-	849	WP_000257196.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
CRT46_RS00265	56443..56820	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
CRT46_RS00270	56823..57644	-	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
CRT46_RS00275	57641..58630	-	990	WP_001520462.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
CRT46_RS00280	58630..59916	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-82)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9482.12 Da
• Isoelectric Point: 10.4134

>T86061 WP_001520461.1 NZ_CP023820:55277-55528 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKF

Nucleotide

• Length: 252 bp

>T86061 NZ_CP023820:55277-55528 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATTCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8560.61 Da
• Isoelectric Point: 5.8062

>AT86061 WP_001520458.1 NZ_CP023820:55041-55274 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLMVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT86061 NZ_CP023820:55041-55274 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTATGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A2T1MJK4