Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 62525..63126 Replicon plasmid pEcIMT16316
Accession NZ_CP023816
Organism Escherichia coli strain IMT16316

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag CRI72_RS25410 Protein ID WP_001216034.1
Coordinates 62746..63126 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag CRI72_RS25405 Protein ID WP_001190712.1
Coordinates 62525..62746 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRI72_RS25395 59506..60789 - 1284 WP_001617890.1 restriction endonuclease subunit S -
CRI72_RS25400 60786..62342 - 1557 WP_001553856.1 type I restriction-modification system subunit M -
CRI72_RS25405 62525..62746 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CRI72_RS25410 62746..63126 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
CRI72_RS25415 63131..63310 + 180 WP_001513661.1 hypothetical protein -
CRI72_RS25420 63338..63697 + 360 WP_001513660.1 hypothetical protein -
CRI72_RS25425 63621..64034 + 414 Protein_74 DDE-type integrase/transposase/recombinase -
CRI72_RS25435 64830..65807 + 978 WP_000361610.1 RepB family plasmid replication initiator protein -
CRI72_RS25440 66092..66832 - 741 WP_001066942.1 tyrosine-type recombinase/integrase -
CRI72_RS25445 66953..67135 - 183 WP_042065278.1 hypothetical protein -
CRI72_RS25455 67478..67785 - 308 Protein_78 type II toxin-antitoxin system ParD family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / tet(A) / blaTEM-1B / mph(A) / sul1 / qacE / aadA5 / dfrA17 / blaCTX-M-15 / aph(6)-Id / aph(3'')-Ib / sul2 / aac(3)-IId - 1..145883 145883
- flank IS/Tn - - 63660..64034 374


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T86055 WP_001216034.1 NZ_CP023816:62746-63126 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T86055 NZ_CP023816:62746-63126 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTAGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT86055 WP_001190712.1 NZ_CP023816:62525-62746 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT86055 NZ_CP023816:62525-62746 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References