Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 2059945..2060557 Replicon chromosome
Accession NZ_CP023815
Organism Escherichia coli strain IMT16316

Toxin (Protein)


Gene name hicA Uniprot ID U9YXE2
Locus tag CRI72_RS10235 Protein ID WP_000833473.1
Coordinates 2059945..2060130 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0A1AGA0
Locus tag CRI72_RS10240 Protein ID WP_022646255.1
Coordinates 2060147..2060557 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRI72_RS10215 2055411..2056706 + 1296 WP_000985736.1 Fic family protein -
CRI72_RS10220 2056814..2058352 + 1539 WP_000183976.1 aldehyde dehydrogenase AldB -
CRI72_RS10225 2058393..2059472 - 1080 WP_000061477.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
CRI72_RS10235 2059945..2060130 + 186 WP_000833473.1 type II toxin-antitoxin system HicA family toxin Toxin
CRI72_RS10240 2060147..2060557 + 411 WP_022646255.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CRI72_RS10245 2060669..2062648 - 1980 WP_022646254.1 glycoside hydrolase family 127 protein -
CRI72_RS10250 2062659..2064014 - 1356 WP_198405091.1 MFS transporter -
CRI72_RS10255 2064047..2064744 + 698 Protein_1869 IS1 family transposase -
CRI72_RS10260 2064755..2065195 - 441 WP_098944925.1 YhcH/YjgK/YiaL family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2055411..2065195 9784


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6800.89 Da        Isoelectric Point: 11.7053

>T86034 WP_000833473.1 NZ_CP023815:2059945-2060130 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T86034 NZ_CP023815:2059945-2060130 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15214.08 Da        Isoelectric Point: 4.4482

>AT86034 WP_022646255.1 NZ_CP023815:2060147-2060557 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALAAHFETLCEMDEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp

>AT86034 NZ_CP023815:2060147-2060557 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGCCGCGCACTTCGAAACCTTATGCGAGATGG
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0A1AGA0

References