Detailed information of TA system TA086030

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1164379..1165177
• Replicon: chromosome
• Accession: NZ_CP023815
• Organism: Escherichia coli strain IMT16316

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1X1M0U2
• Locus tag: CRI72_RS05745
• Protein ID: WP_000854919.1
• Coordinates: 1164379..1164756 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2G4AEZ5
• Locus tag: CRI72_RS05750
• Protein ID: WP_001285608.1
• Coordinates: 1164803..1165177 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRI72_RS05715	1159902..1160081	+	180	WP_001153034.1	hypothetical protein	-
CRI72_RS05720	1160480..1162242	-	1763	Protein_1052	hypothetical protein	-
CRI72_RS26345	1163446..1163592	-	147	WP_001290177.1	hypothetical protein	-
CRI72_RS05735	1163677..1163874	-	198	WP_001327226.1	DUF957 domain-containing protein	-
CRI72_RS05740	1163894..1164382	-	489	WP_000777664.1	hypothetical protein	-
CRI72_RS05745	1164379..1164756	-	378	WP_000854919.1	TA system toxin CbtA family protein	Toxin
CRI72_RS05750	1164803..1165177	-	375	WP_001285608.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
CRI72_RS05755	1165257..1165478	-	222	WP_000692350.1	DUF987 domain-containing protein	-
CRI72_RS05760	1165565..1166041	-	477	WP_001560293.1	RadC family protein	-
CRI72_RS05770	1166056..1166535	-	480	WP_000706978.1	antirestriction protein	-
CRI72_RS05775	1166801..1167619	-	819	WP_001175165.1	DUF945 domain-containing protein	-
CRI72_RS05780	1167709..1167942	-	234	WP_001278293.1	DUF905 family protein	-
CRI72_RS05785	1167948..1168625	-	678	WP_001097302.1	hypothetical protein	-
CRI72_RS05790	1168773..1169453	-	681	WP_001282921.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1154964..1197816	42852

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14037.97 Da
• Isoelectric Point: 7.9086

>T86030 WP_000854919.1 NZ_CP023815:c1164756-1164379 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRSNYRTVNNITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T86030 NZ_CP023815:c1164756-1164379 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13610.37 Da
• Isoelectric Point: 5.5051

>AT86030 WP_001285608.1 NZ_CP023815:c1165177-1164803 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPAITS

Nucleotide

• Length: 375 bp

>AT86030 NZ_CP023815:c1165177-1164803 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCAGATGCATACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACTCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCACCCGCAATCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A1X1M0U2

Antitoxin

• AlphaFold DB: A0A2G4AEZ5