Detailed information of TA system TA086004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 17561..18168
• Replicon: plasmid pER93933.3A.1
• Accession: NZ_CP023781
• Organism: Enterococcus faecium strain Efaecium_ER03933.3A isolate isolate

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: CO993_RS14865
• Protein ID: WP_002321032.1
• Coordinates: 17818..18168 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: CO993_RS14860
• Protein ID: WP_002287514.1
• Coordinates: 17561..17824 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO993_RS14820	12911..13384	+	474	WP_002321302.1	single-stranded DNA-binding protein	-
CO993_RS14825	13750..14010	+	261	WP_002321303.1	hypothetical protein	-
CO993_RS14830	14455..14661	+	207	WP_002296239.1	hypothetical protein	-
CO993_RS14835	14661..14912	+	252	WP_002305766.1	hypothetical protein	-
CO993_RS14840	14928..15344	+	417	WP_002305764.1	hypothetical protein	-
CO993_RS14845	15326..15598	+	273	WP_002348912.1	hypothetical protein	-
CO993_RS14850	15765..16166	+	402	WP_002305759.1	IS200/IS605 family transposase	-
CO993_RS14855	16178..17323	+	1146	WP_002305757.1	IS200/IS605 family element transposase accessory protein TnpB	-
CO993_RS14860	17561..17824	+	264	WP_002287514.1	hypothetical protein	Antitoxin
CO993_RS14865	17818..18168	+	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CO993_RS14870	18192..18806	-	615	WP_002318230.1	recombinase family protein	-
CO993_RS14875	19256..20581	+	1326	WP_002348911.1	Y-family DNA polymerase	-
CO993_RS14880	20574..20924	+	351	WP_002303114.1	hypothetical protein	-
CO993_RS14885	20921..21100	+	180	WP_002322386.1	hypothetical protein	-
CO993_RS14890	21112..21405	+	294	WP_002322251.1	hypothetical protein	-
CO993_RS14895	21631..22449	+	819	WP_002305212.1	ParA family protein	-
CO993_RS14900	22468..22875	+	408	WP_002305774.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..199876	199876

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T86004 WP_002321032.1 NZ_CP023781:17818-18168 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T86004 NZ_CP023781:17818-18168 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT86004 WP_002287514.1 NZ_CP023781:17561-17824 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT86004 NZ_CP023781:17561-17824 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0