Detailed information of TA system TA085999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1730419..1731062
• Replicon: chromosome
• Accession: NZ_CP023778
• Organism: Nocardia terpenica strain NC_YFY_NT001

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CRH09_RS07975
• Protein ID: WP_098693362.1
• Coordinates: 1730643..1731062 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CRH09_RS07970
• Protein ID: WP_098693361.1
• Coordinates: 1730419..1730646 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRH09_RS07950	1725955..1726896	+	942	WP_098693358.1	NAD-dependent epimerase/dehydratase	-
CRH09_RS07955	1727056..1728036	+	981	WP_067582544.1	ABC transporter ATP-binding protein	-
CRH09_RS07960	1728033..1728872	+	840	WP_098693359.1	ABC transporter permease	-
CRH09_RS07965	1728900..1730321	+	1422	WP_098693360.1	NDP-hexose 2,3-dehydratase family protein	-
CRH09_RS07970	1730419..1730646	+	228	WP_098693361.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CRH09_RS07975	1730643..1731062	+	420	WP_098693362.1	PIN domain nuclease	Toxin
CRH09_RS07980	1731119..1731577	-	459	WP_157777352.1	hypothetical protein	-
CRH09_RS07985	1731618..1732100	-	483	WP_157777353.1	hypothetical protein	-
CRH09_RS07990	1732269..1733126	+	858	WP_157777354.1	helix-turn-helix domain-containing protein	-
CRH09_RS07995	1733117..1733473	+	357	WP_098693366.1	DUF397 domain-containing protein	-
CRH09_RS08000	1733551..1734093	+	543	WP_098693367.1	DUF3558 domain-containing protein	-
CRH09_RS08005	1734112..1734483	+	372	WP_098693368.1	hypothetical protein	-
CRH09_RS40175	1734496..1735815	+	1320	WP_157777356.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (31-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-43)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15529.58 Da
• Isoelectric Point: 6.0045

>T85999 WP_098693362.1 NZ_CP023778:1730643-1731062 [Nocardia terpenica]
MTARYLLDKSAGFRMHIEVVDARVGPLMERGLLATCGIADLEAGVSARSREHHQALGDARRYALHYLTTPDEAWERAWEV
QEQLCGRSLHRSVKIPDLVIAAVAEHHGLTVLHYDHDFDRIAEITGQPVEWVVPPGTAD

Nucleotide

• Length: 420 bp

>T85999 NZ_CP023778:1730643-1731062 [Nocardia terpenica]
ATGACCGCGCGGTATCTGCTGGACAAGTCCGCCGGGTTCCGGATGCACATCGAGGTGGTGGACGCGCGGGTCGGTCCGCT
CATGGAACGCGGACTGCTCGCCACGTGCGGGATTGCCGACCTGGAGGCCGGTGTGTCGGCGAGATCCCGAGAACATCACC
AGGCCCTCGGGGACGCCCGCCGCTACGCCTTGCATTACCTGACCACCCCGGACGAAGCGTGGGAACGTGCCTGGGAGGTC
CAAGAGCAGCTGTGCGGCCGCAGCCTGCACCGTAGCGTCAAGATTCCCGATCTCGTCATTGCGGCCGTCGCCGAGCACCA
CGGCCTCACGGTGCTGCACTATGACCACGACTTCGACAGGATCGCCGAGATCACCGGTCAACCCGTCGAATGGGTTGTGC
CACCCGGCACCGCGGATTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8064.11 Da
• Isoelectric Point: 5.0041

>AT85999 WP_098693361.1 NZ_CP023778:1730419-1730646 [Nocardia terpenica]
MTRTMIDLDDDLLARAAKELGTATKKDTVHAALRVALRQSAARNLRAMMAGDPEGLAHEATVNSMWEDGDQAPTA

Nucleotide

• Length: 228 bp

>AT85999 NZ_CP023778:1730419-1730646 [Nocardia terpenica]
ATGACGCGGACGATGATTGATCTTGATGACGACCTGTTGGCTAGGGCCGCGAAGGAGTTGGGTACGGCGACGAAGAAGGA
TACGGTTCACGCGGCTTTGCGGGTTGCGTTGCGCCAGAGCGCCGCGCGGAACCTGCGCGCCATGATGGCCGGAGATCCGG
AGGGCCTAGCGCACGAAGCGACAGTCAACTCGATGTGGGAGGACGGCGACCAGGCACCGACCGCATGA