Detailed information of TA system TA085996

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 766153..766690
• Replicon: chromosome
• Accession: NZ_CP023776
• Organism: Burkholderia pseudomallei strain BPHN1

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: CNX72_RS25400
• Protein ID: WP_009923450.1
• Coordinates: 766153..766422 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: CNX72_RS25405
• Protein ID: WP_004525420.1
• Coordinates: 766406..766690 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNX72_RS38870	761931..762359	-	429	Protein_624	DDE-type integrase/transposase/recombinase	-
CNX72_RS38875	762357..763130	-	774	Protein_625	prohibitin family protein	-
CNX72_RS25370	763526..764179	+	654	WP_004545536.1	hypothetical protein	-
CNX72_RS38325	764365..764583	-	219	WP_004552392.1	hypothetical protein	-
CNX72_RS38330	764631..764789	-	159	WP_004549802.1	hypothetical protein	-
CNX72_RS25380	764786..765028	-	243	WP_004545584.1	hypothetical protein	-
CNX72_RS25390	765166..765297	-	132	WP_009923452.1	bacteriophage protein Gp48	-
CNX72_RS25395	765307..765582	-	276	WP_004552388.1	bacteriophage protein Gp49	-
CNX72_RS25400	766153..766422	+	270	WP_009923450.1	BrnT family toxin	Toxin
CNX72_RS25405	766406..766690	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
CNX72_RS25420	767337..767720	+	384	Protein_634	DDE-type integrase/transposase/recombinase	-
CNX72_RS25425	767683..768111	+	429	Protein_635	IS110 family transposase	-
CNX72_RS25430	768338..768454	-	117	Protein_636	IS3 family transposase	-
CNX72_RS25435	768701..770161	+	1461	WP_004538070.1	metallophosphoesterase	-
CNX72_RS25440	770320..771582	+	1263	WP_017881884.1	c-type cytochrome	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	746033..770161	24128

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10156.52 Da
• Isoelectric Point: 7.1633

>T85996 WP_009923450.1 NZ_CP023776:766153-766422 [Burkholderia pseudomallei]
MDITFDPTKNKTNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T85996 NZ_CP023776:766153-766422 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACAAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT85996 WP_004525420.1 NZ_CP023776:766406-766690 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT85996 NZ_CP023776:766406-766690 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8