Detailed information of TA system TA085993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1232188..1233105
• Replicon: chromosome
• Accession: NZ_CP023748
• Organism: Bacillus velezensis strain LABIM40

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: CQJ38_RS06540
• Protein ID: WP_032866111.1
• Coordinates: 1232359..1233105 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: CQJ38_RS06535
• Protein ID: WP_003154807.1
• Coordinates: 1232188..1232358 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQJ38_RS06500	1227413..1229044	+	1632	WP_060561803.1	pyocin knob domain-containing protein	-
CQJ38_RS06505	1229057..1229428	+	372	WP_060561802.1	hypothetical protein	-
CQJ38_RS06510	1229433..1229630	+	198	WP_059367156.1	XkdX family protein	-
CQJ38_RS06515	1229687..1230447	+	761	Protein_1212	phage portal protein	-
CQJ38_RS06520	1230499..1230762	+	264	WP_098081338.1	hemolysin XhlA family protein	-
CQJ38_RS06525	1230776..1231039	+	264	WP_003154813.1	phage holin	-
CQJ38_RS06530	1231053..1231931	+	879	WP_043021264.1	N-acetylmuramoyl-L-alanine amidase	-
CQJ38_RS06535	1232188..1232358	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
CQJ38_RS06540	1232359..1233105	-	747	WP_032866111.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
CQJ38_RS06545	1233210..1234208	-	999	WP_007407255.1	inorganic phosphate transporter	-
CQJ38_RS06550	1234221..1234838	-	618	WP_003154804.1	DUF47 domain-containing protein	-
CQJ38_RS06555	1235124..1236440	-	1317	WP_007610842.1	amino acid permease	-
CQJ38_RS06560	1236763..1237713	+	951	WP_098081339.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29095.60 Da
• Isoelectric Point: 4.7755

>T85993 WP_032866111.1 NZ_CP023748:c1233105-1232359 [Bacillus velezensis]
MLMFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T85993 NZ_CP023748:c1233105-1232359 [Bacillus velezensis]
ATGCTTATGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGTTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT85993 WP_003154807.1 NZ_CP023748:c1232358-1232188 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT85993 NZ_CP023748:c1232358-1232188 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14