Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 470470..471107 Replicon chromosome
Accession NZ_CP023748
Organism Bacillus velezensis strain LABIM40

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag CQJ38_RS02395 Protein ID WP_003156187.1
Coordinates 470757..471107 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag CQJ38_RS02390 Protein ID WP_003156188.1
Coordinates 470470..470751 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CQJ38_RS02370 466835..467434 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
CQJ38_RS02375 467527..467892 + 366 WP_007410229.1 holo-ACP synthase -
CQJ38_RS02380 468057..469064 + 1008 WP_098081024.1 outer membrane lipoprotein carrier protein LolA -
CQJ38_RS02385 469181..470350 + 1170 WP_098081026.1 alanine racemase -
CQJ38_RS02390 470470..470751 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
CQJ38_RS02395 470757..471107 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CQJ38_RS02400 471225..472046 + 822 WP_003156182.1 STAS domain-containing protein -
CQJ38_RS02405 472051..472416 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
CQJ38_RS02410 472419..472820 + 402 WP_003156178.1 anti-sigma regulatory factor -
CQJ38_RS02415 472832..473839 + 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
CQJ38_RS02420 473903..474232 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
CQJ38_RS02425 474229..474711 + 483 WP_007609591.1 anti-sigma B factor RsbW -
CQJ38_RS02430 474677..475465 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
CQJ38_RS02435 475465..476067 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T85992 WP_003156187.1 NZ_CP023748:470757-471107 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T85992 NZ_CP023748:470757-471107 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT85992 WP_003156188.1 NZ_CP023748:470470-470751 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT85992 NZ_CP023748:470470-470751 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References