Detailed information of TA system TA085992

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 470470..471107
• Replicon: chromosome
• Accession: NZ_CP023748
• Organism: Bacillus velezensis strain LABIM40

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: CQJ38_RS02395
• Protein ID: WP_003156187.1
• Coordinates: 470757..471107 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: CQJ38_RS02390
• Protein ID: WP_003156188.1
• Coordinates: 470470..470751 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQJ38_RS02370	466835..467434	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
CQJ38_RS02375	467527..467892	+	366	WP_007410229.1	holo-ACP synthase	-
CQJ38_RS02380	468057..469064	+	1008	WP_098081024.1	outer membrane lipoprotein carrier protein LolA	-
CQJ38_RS02385	469181..470350	+	1170	WP_098081026.1	alanine racemase	-
CQJ38_RS02390	470470..470751	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
CQJ38_RS02395	470757..471107	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
CQJ38_RS02400	471225..472046	+	822	WP_003156182.1	STAS domain-containing protein	-
CQJ38_RS02405	472051..472416	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
CQJ38_RS02410	472419..472820	+	402	WP_003156178.1	anti-sigma regulatory factor	-
CQJ38_RS02415	472832..473839	+	1008	WP_007410233.1	PP2C family protein-serine/threonine phosphatase	-
CQJ38_RS02420	473903..474232	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
CQJ38_RS02425	474229..474711	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
CQJ38_RS02430	474677..475465	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
CQJ38_RS02435	475465..476067	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T85992 WP_003156187.1 NZ_CP023748:470757-471107 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T85992 NZ_CP023748:470757-471107 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT85992 WP_003156188.1 NZ_CP023748:470470-470751 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT85992 NZ_CP023748:470470-470751 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5