Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2822952..2823748 Replicon chromosome
Accession NZ_CP023737
Organism Methylosinus trichosporium OB3b

Toxin (Protein)


Gene name TacT3 Uniprot ID A0A1A6FN69
Locus tag CQW49_RS13540 Protein ID WP_003608950.1
Coordinates 2822952..2823476 (-) Length 175 a.a.

Antitoxin (Protein)


Gene name TacA3 Uniprot ID A0A1A6FQL2
Locus tag CQW49_RS13545 Protein ID WP_003608948.1
Coordinates 2823473..2823748 (-) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CQW49_RS13520 2818495..2819358 - 864 WP_003608957.1 triphosphoribosyl-dephospho-CoA synthase -
CQW49_RS13525 2819351..2820316 - 966 WP_003608955.1 RimK family alpha-L-glutamate ligase -
CQW49_RS13530 2820270..2821247 - 978 WP_003608953.1 methenyltetrahydromethanopterin cyclohydrolase -
CQW49_RS13535 2821764..2822249 + 486 WP_155931266.1 hypothetical protein -
CQW49_RS13540 2822952..2823476 - 525 WP_003608950.1 GNAT family N-acetyltransferase Toxin
CQW49_RS13545 2823473..2823748 - 276 WP_003608948.1 DUF1778 domain-containing protein Antitoxin
CQW49_RS13550 2823901..2825124 - 1224 WP_003608946.1 alpha/beta hydrolase -
CQW49_RS13555 2825282..2825854 + 573 WP_003608944.1 L,D-transpeptidase -
CQW49_RS13560 2826239..2827024 + 786 WP_099831891.1 3-methyl-2-oxobutanoate hydroxymethyltransferase -
CQW49_RS13565 2827021..2828007 + 987 WP_003608940.1 ferrochelatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2816443..2972913 156470


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(31-119)

Antitoxin

Vibrio phage ICP1, Orf50

(10-85)


Sequences

Toxin        


Download         Length: 175 a.a.        Molecular weight: 19142.95 Da        Isoelectric Point: 8.6771

>T85981 WP_003608950.1 NZ_CP023737:c2823476-2822952 [Methylosinus trichosporium OB3b]
MTALEWEEVSLGKIHDRNAFHCGEDHLDTYLKRYARQNHESGGAKCFVAAARDAPTRVLGFYTLSPASIEFARAPAVATR
GLGRYEVPVYRLGRLAVDRSVQGRGLGGRLLLRAAERCMAVAQQVGGVALLIDAKTEMVAEWYENYGALRLLDAPRSLIL
PFAVVAEAVRQGER

Download         Length: 525 bp

>T85981 NZ_CP023737:c2823476-2822952 [Methylosinus trichosporium OB3b]
ATGACGGCGCTGGAGTGGGAAGAAGTTTCGCTCGGCAAGATCCACGACCGCAACGCATTCCACTGCGGCGAGGATCATCT
CGATACCTATTTGAAACGCTACGCGCGCCAAAACCATGAAAGCGGCGGCGCGAAATGCTTCGTTGCGGCGGCGCGCGACG
CCCCCACGCGCGTTCTCGGCTTCTATACACTGAGCCCCGCGTCGATCGAATTTGCGCGCGCCCCTGCCGTTGCGACGCGC
GGCCTCGGTCGCTACGAGGTCCCGGTGTATCGGCTCGGCCGCTTGGCCGTCGATCGCAGCGTGCAAGGCCGGGGGCTCGG
CGGCCGCCTGCTGCTGCGCGCCGCGGAACGCTGCATGGCCGTCGCGCAGCAAGTCGGCGGCGTGGCTCTGCTGATCGACG
CCAAGACCGAAATGGTCGCGGAATGGTACGAAAACTACGGGGCACTACGTCTCCTCGATGCGCCGCGCTCGCTCATCCTG
CCCTTCGCCGTGGTGGCCGAAGCGGTGAGGCAAGGGGAGCGATGA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10167.76 Da        Isoelectric Point: 8.8518

>AT85981 WP_003608948.1 NZ_CP023737:c2823748-2823473 [Methylosinus trichosporium OB3b]
MPREHAETGRVELRLKPEDKAVLARAAALERLDLTSFILRAALPRAQDVIAQSERLKLSERDSLRVLDLLENPPAPTERL
TRAAKGGFVLE

Download         Length: 276 bp

>AT85981 NZ_CP023737:c2823748-2823473 [Methylosinus trichosporium OB3b]
ATGCCCCGCGAACATGCCGAAACTGGCCGGGTCGAGCTGCGGCTGAAGCCGGAGGACAAGGCTGTGCTGGCGCGCGCGGC
CGCGCTCGAGCGGCTCGATCTGACCAGCTTCATCCTGCGCGCCGCGCTGCCGCGCGCGCAGGATGTCATCGCGCAGTCCG
AGAGGCTGAAGCTGTCCGAGCGCGATTCCCTGCGCGTGCTCGACCTCTTGGAAAACCCCCCGGCCCCCACGGAGCGACTG
ACGCGCGCGGCGAAGGGCGGCTTCGTGCTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1A6FN69

Antitoxin

Source ID Structure
AlphaFold DB A0A1A6FQL2

References