Detailed information of TA system TA085980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2503159..2503841
• Replicon: chromosome
• Accession: NZ_CP023737
• Organism: Methylosinus trichosporium OB3b

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A1A6FQN5
• Locus tag: CQW49_RS12100
• Protein ID: WP_003612262.1
• Coordinates: 2503431..2503841 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CQW49_RS12095
• Protein ID: WP_003612263.1
• Coordinates: 2503159..2503422 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQW49_RS12075	2499014..2500036	-	1023	WP_024749740.1	hypothetical protein	-
CQW49_RS12080	2500059..2500631	+	573	WP_003612266.1	isochorismatase family protein	-
CQW49_RS12085	2500708..2501109	+	402	WP_003612265.1	DUF3775 domain-containing protein	-
CQW49_RS12090	2501230..2503086	+	1857	WP_003612264.1	cation:proton antiporter	-
CQW49_RS12095	2503159..2503422	+	264	WP_003612263.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CQW49_RS12100	2503431..2503841	+	411	WP_003612262.1	type II toxin-antitoxin system VapC family toxin	Toxin
CQW49_RS12105	2504036..2504368	-	333	WP_003612261.1	hypothetical protein	-
CQW49_RS12115	2505930..2507039	-	1110	WP_003612259.1	helix-turn-helix transcriptional regulator	-
CQW49_RS12120	2507109..2508047	-	939	WP_003612258.1	outer membrane beta-barrel protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

• SpoVT-AbrB domain (12-48)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14966.38 Da
• Isoelectric Point: 6.7211

>T85980 WP_003612262.1 NZ_CP023737:2503431-2503841 [Methylosinus trichosporium OB3b]
MFFLDTNVVIAVMTRRAPHLVARIEAELAHGTPLLLSTIVLYELEYGACKSAHPERNLARIGDFLVSVAAIVPLDVEDAR
EAGDIRAFLEAKGTPIGPYDVLIAAQARRRRIALVTGNRREFDRVPGLVMTDWTAA

Nucleotide

• Length: 411 bp

>T85980 NZ_CP023737:2503431-2503841 [Methylosinus trichosporium OB3b]
ATGTTTTTTCTCGATACGAATGTCGTGATCGCAGTCATGACGCGCCGCGCGCCCCATCTCGTGGCGCGCATCGAGGCCGA
ACTCGCGCACGGGACGCCGTTGCTGCTGTCGACGATCGTTCTCTATGAACTCGAATATGGGGCGTGCAAGAGCGCTCATC
CCGAGCGAAACCTCGCGCGGATCGGAGATTTCCTCGTCAGCGTCGCGGCTATCGTTCCACTCGACGTCGAGGACGCGCGC
GAAGCCGGCGACATCCGAGCATTTCTCGAAGCGAAAGGAACGCCGATCGGCCCCTATGACGTGTTGATCGCCGCCCAGGC
GCGACGCCGGCGTATTGCGCTCGTTACCGGCAACCGCCGCGAGTTCGACCGCGTGCCCGGTCTCGTCATGACCGATTGGA
CCGCCGCCTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10167.66 Da
• Isoelectric Point: 5.1875

>AT85980 WP_003612263.1 NZ_CP023737:2503159-2503422 [Methylosinus trichosporium OB3b]
MHEQNKATAKLFMHGRSQAVRLPKEFRFEGSQVRVSRIGDKVILEPMEKPPFDVKAWRAKLDAYLDIDFPEIERIPPLEP
DEDVTFD

Nucleotide

• Length: 264 bp

>AT85980 NZ_CP023737:2503159-2503422 [Methylosinus trichosporium OB3b]
ATGCACGAGCAGAACAAAGCGACGGCCAAGCTGTTCATGCACGGCCGCAGTCAGGCCGTGCGTCTGCCCAAGGAGTTCCG
CTTCGAGGGCAGCCAGGTGCGGGTGAGCCGGATCGGCGACAAGGTGATTTTGGAGCCCATGGAAAAGCCGCCCTTCGACG
TGAAGGCGTGGCGCGCGAAGCTCGACGCCTATCTGGACATTGATTTTCCCGAGATCGAAAGGATTCCGCCGCTCGAACCC
GACGAAGACGTCACTTTCGACTGA

Structures

Toxin

• AlphaFold DB: A0A1A6FQN5