Detailed information of TA system TA085963

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 50897..51540
• Replicon: plasmid unnamed3
• Accession: NZ_CP023725
• Organism: Klebsiella pneumoniae strain TVGHCRE225

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: CO233_RS31275
• Protein ID: WP_001044770.1
• Coordinates: 51124..51540 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: CO233_RS31270
• Protein ID: WP_001261282.1
• Coordinates: 50897..51127 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO233_RS31235	46120..46899	-	780	WP_013214009.1	site-specific integrase	-
CO233_RS31240	47083..48087	-	1005	WP_011977814.1	hypothetical protein	-
CO233_RS31245	48117..48320	-	204	WP_011977813.1	hypothetical protein	-
CO233_RS31250	48366..48887	-	522	WP_013214008.1	hypothetical protein	-
CO233_RS31255	48945..49337	-	393	WP_011977811.1	hypothetical protein	-
CO233_RS31260	49374..50318	-	945	WP_011977810.1	hypothetical protein	-
CO233_RS33300	50479..50940	-	462	WP_014343465.1	hypothetical protein	-
CO233_RS31270	50897..51127	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CO233_RS31275	51124..51540	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
CO233_RS31280	51614..53176	+	1563	WP_004206609.1	AAA family ATPase	-
CO233_RS31285	53161..54183	+	1023	WP_000361404.1	helicase UvrD	-
CO233_RS31290	54439..55136	+	698	Protein_73	IS1 family transposase	-
-	55337..55396	-	60	NuclAT_0	-	-
-	55337..55396	-	60	NuclAT_0	-	-
-	55337..55396	-	60	NuclAT_0	-	-
-	55337..55396	-	60	NuclAT_0	-	-
CO233_RS31300	55441..55590	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	-
CO233_RS31305	55874..56122	+	249	WP_000083837.1	replication regulatory protein RepA	-
CO233_RS31315	56367..56441	+	75	WP_001375168.1	RepA leader peptide Tap	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-2	-	1..103454	103454
	-	flank	IS/Tn	-	-	54633..55136	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T85963 WP_001044770.1 NZ_CP023725:51124-51540 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T85963 NZ_CP023725:51124-51540 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT85963 WP_001261282.1 NZ_CP023725:50897-51127 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT85963 NZ_CP023725:50897-51127 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3