Detailed information of TA system TA085961

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 140742..141412
• Replicon: plasmid unnamed1
• Accession: NZ_CP023723
• Organism: Klebsiella pneumoniae strain TVGHCRE225

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: CO233_RS28995
• Protein ID: WP_004213072.1
• Coordinates: 140742..141185 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: CO233_RS29000
• Protein ID: WP_004213073.1
• Coordinates: 141182..141412 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO233_RS28945	136154..136429	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
CO233_RS28950	136492..136983	+	492	WP_011251327.1	DM13 domain-containing protein	-
CO233_RS28955	137032..137952	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
CO233_RS28965	138043..138449	+	407	Protein_144	GAF domain-containing protein	-
CO233_RS33565	138963..139598	-	636	WP_165452888.1	response regulator	-
CO233_RS28985	140015..140308	+	294	Protein_146	transposase	-
CO233_RS28990	140312..140728	+	417	Protein_147	restriction endonuclease	-
CO233_RS28995	140742..141185	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
CO233_RS29000	141182..141412	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CO233_RS29005	142020..143153	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
CO233_RS29010	143169..143462	+	294	WP_004213076.1	hypothetical protein	-
CO233_RS29015	143452..143658	-	207	WP_004213077.1	hypothetical protein	-
CO233_RS29020	144010..144300	+	291	WP_004213078.1	hypothetical protein	-
CO233_RS29025	144290..145189	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA	1..297984	297984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T85961 WP_004213072.1 NZ_CP023723:c141185-140742 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T85961 NZ_CP023723:c141185-140742 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT85961 WP_004213073.1 NZ_CP023723:c141412-141182 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT85961 NZ_CP023723:c141412-141182 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620