Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 5235752..5236406 Replicon chromosome
Accession NZ_CP023722
Organism Klebsiella pneumoniae strain TVGHCRE225

Toxin (Protein)


Gene name cptA Uniprot ID R4YHX2
Locus tag CO233_RS26540 Protein ID WP_004144731.1
Coordinates 5235752..5236159 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag CO233_RS26545 Protein ID WP_002916312.1
Coordinates 5236140..5236406 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO233_RS26520 5231752..5233485 - 1734 WP_004151783.1 single-stranded-DNA-specific exonuclease RecJ -
CO233_RS26525 5233491..5234204 - 714 WP_002916301.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CO233_RS26530 5234227..5235123 - 897 WP_004144729.1 site-specific tyrosine recombinase XerD -
CO233_RS26535 5235224..5235745 + 522 WP_002916308.1 flavodoxin FldB -
CO233_RS26540 5235752..5236159 - 408 WP_004144731.1 protein YgfX Toxin
CO233_RS26545 5236140..5236406 - 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
CO233_RS26550 5236652..5237635 + 984 WP_002916313.1 tRNA-modifying protein YgfZ -
CO233_RS26555 5237786..5238445 - 660 WP_002916317.1 hemolysin III family protein -
CO233_RS26560 5238609..5238920 - 312 WP_002916319.1 N(4)-acetylcytidine aminohydrolase -
CO233_RS26565 5238970..5239698 + 729 WP_002916321.1 MurR/RpiR family transcriptional regulator -
CO233_RS26570 5239817..5241250 + 1434 WP_002916322.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15936.69 Da        Isoelectric Point: 11.4778

>T85957 WP_004144731.1 NZ_CP023722:c5236159-5235752 [Klebsiella pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNAE
WEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 408 bp

>T85957 NZ_CP023722:c5236159-5235752 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT85957 WP_002916312.1 NZ_CP023722:c5236406-5236140 [Klebsiella pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT85957 NZ_CP023722:c5236406-5236140 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378E4P6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References