Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1939571..1940190 Replicon chromosome
Accession NZ_CP023722
Organism Klebsiella pneumoniae strain TVGHCRE225

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CO233_RS09835 Protein ID WP_002892050.1
Coordinates 1939571..1939789 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag CO233_RS09840 Protein ID WP_002892066.1
Coordinates 1939816..1940190 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO233_RS09805 1935618..1935881 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
CO233_RS09810 1935881..1936021 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
CO233_RS09815 1936018..1936716 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
CO233_RS09820 1936817..1938268 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
CO233_RS09825 1938243..1938713 - 471 WP_002892026.1 YlaC family protein -
CO233_RS32205 1938734..1938874 + 141 WP_004147370.1 hypothetical protein -
CO233_RS09830 1938846..1939412 - 567 WP_002892030.1 maltose O-acetyltransferase -
CO233_RS09835 1939571..1939789 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CO233_RS09840 1939816..1940190 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
CO233_RS09845 1940676..1943822 - 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit -
CO233_RS09850 1943845..1945038 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T85947 WP_002892050.1 NZ_CP023722:c1939789-1939571 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T85947 NZ_CP023722:c1939789-1939571 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT85947 WP_002892066.1 NZ_CP023722:c1940190-1939816 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT85947 NZ_CP023722:c1940190-1939816 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References