Detailed information of TA system TA085935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 42851..43404
• Replicon: plasmid unnamed1
• Accession: NZ_CP023712
• Organism: Rhodococcus ruber strain YC-YT1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: YT1_RS00255
• Protein ID: WP_119699076.1
• Coordinates: 43123..43404 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: YT1_RS00250
• Protein ID: WP_119699075.1
• Coordinates: 42851..43126 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
YT1_RS00235	37995..39349	+	1355	Protein_46	3' terminal RNA ribose 2'-O-methyltransferase Hen1	-
YT1_RS00240	39346..41856	+	2511	WP_119699074.1	polynucleotide kinase-phosphatase	-
YT1_RS00245	42005..42438	+	434	Protein_48	nitronate monooxygenase	-
YT1_RS00250	42851..43126	+	276	WP_119699075.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
YT1_RS00255	43123..43404	+	282	WP_119699076.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
YT1_RS00260	44153..44800	-	648	WP_119699077.1	TetR/AcrR family transcriptional regulator	-
YT1_RS00265	45107..46690	+	1584	WP_119699078.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..159746	159746

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (14-91)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10569.11 Da
• Isoelectric Point: 10.2269

>T85935 WP_119699076.1 NZ_CP023712:43123-43404 [Rhodococcus ruber]
MTEQTPVPYDVEIASPARRALSRLPGRVAHAVVEFISGPLAGNPHRLSKPLRNELEGLRSARRGDYRVLLRVDDEHRTVL
VVDIDHRAHIYRI

Nucleotide

• Length: 282 bp

>T85935 NZ_CP023712:43123-43404 [Rhodococcus ruber]
ATGACCGAGCAGACGCCGGTACCCTACGACGTCGAGATCGCCTCACCGGCCCGCCGAGCCCTGTCCCGGCTACCCGGACG
GGTCGCGCACGCGGTCGTCGAGTTCATCTCCGGCCCGTTGGCAGGAAATCCGCACCGGCTGAGCAAACCGCTGCGCAACG
AGCTCGAGGGCCTGCGCAGCGCCCGTCGCGGTGACTACCGCGTCCTGTTGCGCGTCGACGACGAACACCGCACCGTCCTC
GTTGTCGACATCGACCACCGTGCTCACATCTACCGAATCTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 9844.08 Da
• Isoelectric Point: 4.8210

>AT85935 WP_119699075.1 NZ_CP023712:42851-43126 [Rhodococcus ruber]
MMTVPLGEAKDKLSALVDRAETTHDIITITRHGKPAAVLMSADDLESLHETLYWLSRPGTAEAVAVADGEYAAGRTTSLD
ELRAEHGFPPR

Nucleotide

• Length: 276 bp

>AT85935 NZ_CP023712:42851-43126 [Rhodococcus ruber]
ATGATGACGGTACCGCTCGGTGAAGCCAAGGACAAGCTCTCGGCGCTGGTCGACCGTGCCGAAACCACGCACGACATCAT
CACGATCACCCGGCACGGCAAACCGGCTGCGGTCCTGATGTCGGCCGACGATCTGGAATCGCTGCACGAGACGCTCTACT
GGCTCTCGCGCCCCGGCACCGCCGAGGCGGTCGCGGTCGCCGACGGCGAGTACGCCGCCGGCCGGACCACCTCGCTCGAC
GAGCTGCGCGCCGAGCACGGCTTCCCGCCTCGATGA