Detailed information of TA system TA085933

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 4873837..4874475
• Replicon: chromosome
• Accession: NZ_CP023711
• Organism: Paenibacillus polymyxa strain C12

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: CPY53_RS21060
• Protein ID: WP_007429335.1
• Coordinates: 4874125..4874475 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: CPY53_RS21055
• Protein ID: WP_242210639.1
• Coordinates: 4873837..4874118 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPY53_RS21035 (CPY53_21050)	4868854..4869825	-	972	WP_031463106.1	ABC transporter substrate-binding protein	-
CPY53_RS21040 (CPY53_21055)	4870060..4870692	+	633	WP_031463107.1	LysE family transporter	-
CPY53_RS21045 (CPY53_21060)	4870803..4872080	+	1278	WP_242210638.1	outer membrane lipoprotein-sorting protein	-
CPY53_RS21050 (CPY53_21065)	4872347..4873543	+	1197	WP_031463109.1	alanine racemase	-
CPY53_RS21055 (CPY53_21070)	4873837..4874118	+	282	WP_242210639.1	ribbon-helix-helix protein, CopG family	Antitoxin
CPY53_RS21060 (CPY53_21075)	4874125..4874475	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CPY53_RS21065 (CPY53_21080)	4874654..4876876	+	2223	WP_031463110.1	Tex family protein	-
CPY53_RS21070 (CPY53_21085)	4876980..4877114	-	135	WP_016821163.1	cortex morphogenetic protein CmpA	-
CPY53_RS21075 (CPY53_21090)	4877257..4877769	+	513	WP_016821162.1	SprT family protein	-
CPY53_RS21090 (CPY53_21105)	4878148..4879023	-	876	WP_017426527.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T85933 WP_007429335.1 NZ_CP023711:4874125-4874475 [Paenibacillus polymyxa]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T85933 NZ_CP023711:4874125-4874475 [Paenibacillus polymyxa]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCTGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTTAGCCCTACCGTGATCGTAGCAGCCATCACAGCACAGATTCAGAAGG
CAAAGCTGCCTACACACGTGGAAATCGACGCGGCGACGCATGGCTTTGATCGTGATTCTGTTGTTCTTCTGGAGCAAATC
CGGACGATCGACAAGCAAAGGCTTACCGACAAAATCACCCATTTGGATGAAGAAACCATGCGCAAAGTGAGCGATTCGCT
ACAGATCAGTCTCGGTTTGATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10735.39 Da
• Isoelectric Point: 9.5900

>AT85933 WP_242210639.1 NZ_CP023711:4873837-4874118 [Paenibacillus polymyxa]
MANLQNTKRIMISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEE
DANSTLGRTVSRV

Nucleotide

• Length: 282 bp

>AT85933 NZ_CP023711:4873837-4874118 [Paenibacillus polymyxa]
TTGGCCAATTTGCAGAACACCAAAAGAATAATGATCAGTTTGCCAGATCATCTCTTGCAGGAAGTGGACGGGATCGTAGC
TATGGAGAATTCCAACCGCAGTGAATTTATCAGGCAGGCAATGAAGCTTTATGTAGGTGAACGTAAAAAACGTTACATTC
GTGAAGCTATGCAGCGTGGATACATGGAAATGGCTAAAATTAACCTGACCATGGCATCTGAAGCCTTTCACGCGGAGGAA
GATGCAAACAGCACCTTGGGCCGTACAGTTAGCAGGGTGTAA

Structures

Toxin

• AlphaFold DB: H6CFY1